南开大学学报（自然科学版）ActaScientiarumNaturaliumUniversitatisNankaiensisVol.56№1Feb.2023第56卷第1期2023年2月ArticleID:0465-7942(2023)01-0026-09ResearchontheMechanismofGinkgoFoliumtoTreatGlioblastomaYangFu1,2,WangLiuli3,LiJinxing2,SunAigang2,FuYao4,WangZengyong4,WangMingguang2(1.GuangzhouUniversityofChineseMedicine,Guangzhou510006,China;2.DepartmentofNeurosurgery,LinyiPeople'sHospital,Linyin371300,China;3.LanshanMaternalandChildHealthHospital,Linyi276003,China;4.CentralLaboratory,LinyiPeople’sHospital,Linyin371300,China)Abstract:Glioblastoma(GBM)isamalignanttumorwithahighincidencerateandhighinvasioninhu-mancentralnervoussystemtumors.However,thecausesoftheoccurrenceanddevelopmentofglioblasto-maarenotclear.AlthoughGinkgoFolium(GF)extractcanreducethegrowthofU-87gliomacelllinesinnudemice,theactionmechanismremainsunclear.Inthisstudy,networkpharmacology,moleculardock-ing,andcellexperimentwereutilizedtoexploretheunderlyingmolecularmechanismofGFintreatingGBM.TheeffectiveingredientsandactiontargetsofGFwereobtainedfromtheTCMSPdatabase.STRINGdatabasewasappliedforproteininteractionanalysisandCytoscapesoftwarewasusedtobuildthe"compound-target-disease"networkandPPInetwork.TheRlanguagewasadoptedasgeneontology(GO)analysisandKyotoEncyclopediaofGenesandGenomes(KEGG)pathwayanalysis.AutoDocksoft-warewasusedformoleculardockingverification.AcellexperimentwasemployedtoverifytheinhibitionofGBMbytheeffectivecompoundsinGF.Theresultsshowedthat27potentialeffectivecomponentsand112potentialtargetsofGFwereselected.7435targetsofGBMwerescreenedfromsixdatabases,and98overlappingtargetswereobtained.Inactivecomponent-targetnetwork,quercetin,kaempferol,stigmas-terol,andluteolinweretheimportantactivecomponents.InPPInetwork,ESR1,RELA,FOS,CCD1,EGFR,AR,HIF1A,MAPK8,NCOA1,andMDM2werethecoretargets.GOandKEGGanalysesshowedthatthemainpathwaysofGFintreatingGBMinvolvedPI3K-Akt,MAPK,andTNFsignalingpathway.Theresultsofmoleculardockingverifiedthegoodbindingactivityofthekeypharmacodynamicmoleculeswiththecoretar...
