肿瘤防治研究2023年第50卷第1期CancerResPrevTreat,2023,Vol.50,No.1·18·doi:10.3971/j.issn.1000-8578.2023.22.0741胰腺癌中OASL的表达及其对癌细胞增殖和迁移的影响张人丹1，赵春艳2，姚佳欣1，胡先华1，母波1OASLExpressioninPancreaticCancerandItsEffectonProliferationandMigrationofPancreaticCancerCellsZHANGRendan1,ZHAOChunyan2,YAOJiaxin1,HUXianhua1,MUBo11.SchoolofBasicMedicalSciencesandForensicMedicine,NorthSichuanMedicalCollege,Nanchong637000,China;2.SichuanKeyLaboratoryofMedicalImaging,NorthSichuanMedicalCollege,Nanchong637000,ChinaCorrespondingAuthor:MUBo,E-mail:mubo2019@nsmc.edu.cnAbstract:ObjectiveToexploretheeffectofOASLexpressionontheproliferationandmigrationofpancreaticcancercells.MethodsTheGEPIAdatabasewasusedtoanalyzethedifferencesinOASLexpressioninpancreaticcancertissuesandnormalpancreatictissues.TheTIMERdatabasewasusedtoanalyzetherelationshipbetweenOASLexpressionandpatientsurvival.TheTCGAdatabasewasusedtoanalyzethecorrelationofOASLexpressionwiththeclinicopathologicalparametersofpancreaticcancer.shRNAwasusedtoknockdowntheexpressionofOASLgeneinpancreaticcancerpanc-1cells.LentiviruseswereusedtooverexpresstheOASLgeneinpancreaticcancercells.MTTassaywasusedtoevaluatetheirproliferationability,andscratchandTranswellexperimentswereusedtoevaluatetheirmigrationability.Westernblotexperimentswereusedtodetectchangesinproteinsrelatedtotumorproliferation,migration,andinvasion.ResultsOASLexpressioninthepancreaticcancergroupwassignificantlyhigherthanthatinnormalpancreatictissue(P<0.05),andpatientswithhighOASLexpressioninpancreaticcancerpatientshadworseOSthanpatientswithlowexpression(P<0.05).AfterOASLgeneknockdown,theproliferationandmigrationabilitiesofpanc-1cellswereinhibited,whereastheoverexpressionofOASLgenepromotedtheproliferationandmigrationabilityofpanc-1cells.WesternblotexperimentsshowedthatafterOASLknockdown,p-STAT3proteinexpressionincreased,whereasSTAT3andBAKproteinexpressionsdecreased.AfterOASLoverexpression,p-STAT3proteinexpressiondecreased,andSTAT3andBAKproteinexpressi...
