农业环境科学学报JournalofAgro⁃EnvironmentScience2022，41（12）:2711-27212022年12月林辉，成琪璐，邹平，等.长期植稻年限序列土壤耐药组演替特征[J].农业环境科学学报,2022,41（12）：2711-2721.LINH,CHENGQL,ZOUP,etal.Successioncharacteristicsofsoilresistomeinpaddysoilchronosequences[J].JournalofAgro-EnvironmentScience,2022,41（12）：2711-2721.开放科学OSID长期植稻年限序列土壤耐药组演替特征林辉，成琪璐，邹平，孙万春，俞巧钢，叶静，陈照明，马军伟*（农产品质量安全危害因子与风险防控国家重点实验室，浙江省农业科学院环境资源与土壤肥料研究所，杭州310021）SuccessioncharacteristicsofsoilresistomeinpaddysoilchronosequencesLINHui,CHENGQilu,ZOUPing,SUNWanchun,YUQiaogang,YEJing,CHENZhaoming,MAJunwei*（StateKeyLaboratoryforManagingBioticandChemicalThreatstotheQualityandSafetyofAgro-products,InstituteofEnvironment,Resource,Soil&Fertilizer,ZhejiangAcademyofAgriculturalSciences,Hangzhou310021,China）Abstract：TwopaddysoilchronosequencesinthesouthbankofHangzhouBay（Shangyu：30–2000a;Cixi：9–2000a）,whichhadbeenpreviouslyidentified,wereusedtocharacterizethesuccessionofpaddysoilresistomeduringlong-termriceplanting,toexpandthescientificunderstandingoftheformationanddevelopmentofpaddysoil.Theoccurrence,abundances,anddiversityof320antibioticresistancegenes（ARGs）indifferentsoildepths（arablelayer,plowpan,andC-horizon）andtheirsuccessionwerestudiedusinghigh-throughputquantitativepolymerasechainreaction（HT-qPCR）.Resultsindicatedthat289ARGsweredetectedintwopaddysoilchronosequenceswithaminoglycosidesandmultidrugresistantARGsasthemostdiverse.Atotalof181ARGsweresharedbetweenbothsoilchronosequences.Theprincipalcoordinateanalysis（PCoA）resultshowedthatthecompositionofARGsinShangyuandCixipaddysoilsequencesweresignificantlydifferent,partlyreflectedinthenumberofARGssubtypes.Cixihad108uniqueARGs,whichdidnot收稿日期：2022-10-12录用日期：2022-11-22作者简介：林辉（1986—），女，浙江温州人，博士，研究员，主要从事环境污染物的生物降解与修复研究。E-mail：lin82774872@163.com*通信作者：马军伟E-mail：majw@zaas....
