针刺百会穴对产后抑郁小鼠行...MDAR相关蛋白表达的影响_刘晶.pdf

现代生物医学进展Progress in Modern Biomedicine Vol.23NO.1JAN.2023doi:10.13241/ki.pmb.2023.01.004针刺百会穴对产后抑郁小鼠行为学改变和海马区NMDAR 相关蛋白表达的影响*刘晶1胡喜姣2石婷婷3王婧3常萍3（1 黑龙江中医药大学研究生学院 黑龙江 哈尔滨 150040；2 黑龙江中医药大学附属第二医院妇产科 黑龙江 哈尔滨 150001；3 齐齐哈尔市中医医院妇产科 黑龙江 齐齐哈尔 161000）摘要 目的：研究针刺百会穴对产后抑郁小鼠行为学改变和海马区 N-甲基-D-天冬氨酸受体（NMDAR）相关蛋白表达的影响。方法：30 只 C57BL/6 母鼠被随机分为对照组、模型组和治疗组，每组 30 只。模型组和治疗组小鼠在妊娠期间通过皮下注射地塞米松磷酸钠建立产后抑郁小鼠模型，对照组小鼠皮下注射等量的生理盐水作为对照。治疗组小鼠分娩后通过针刺百会穴治疗 14 天，模型组和对照组小鼠不进行治疗。比较三组小鼠 24 h 食物消耗量和体重，黑白箱实验中白箱停留时间和黑白箱穿梭次数，以及强迫游泳实验不动状态时间和悬尾实验中悬尾不动时间。同时，通过免疫印记法检测三组小鼠海马去 NMDA 受体（NR2A 和NR2B）、cAMP 结合蛋白(CREB)和钙调蛋白激酶 II(CaMKII)蛋白表达水平。结果：治疗前，产后抑郁小鼠 24 h 食物消耗量、体重、白箱停留时间、黑白箱穿梭次数以及海马区 CREB 蛋白表达水平均显著低于对照组小鼠（P0.05），而游泳不同状态时间、悬尾不动时间和海马区 NR2A、NR2B、cAMP 蛋白表达水平均显著高于对照组小鼠（P0.05）。治疗后，针刺百会穴治疗组小鼠 24 h 食物消耗量、体重、白箱停留时间和黑白箱穿梭次数以及海马区 CREB 蛋白表达水平均显著高于模型组小鼠（P0.05），而游泳不同状态时间、悬尾不动时间和海马区 NR2A、NR2B、cAMP 蛋白表达水平均显著低于模型组小鼠（P0.05）。结论：针刺百会穴可以显著改善产后抑郁小鼠行为学情况，提高其运动能力，其可能与影响产后抑郁小鼠 NMDAR 相关蛋白表达有关。关键词：百会穴；针刺；产后抑郁；NMDAR 相关蛋白中图分类号：R-33；R245；R714.6文献标识码：A文章编号：1673-6273（2023）01-20-05Effects of Acupuncture at Baihui Point on Behavioral Changes andExpression of NMDAR-related Proteins in Hippocampus of Mice withPostpartum Depression*LIU Jing1,HU Xi-jiao2,SHI Ting-ting3,WANG Jing3,CHANG Ping3(1 Graduate School of Heilongjiang University of Traditional Chinese Medicine,Harbin,Heilongjiang,150040,China;2 Department of Obstetrics and Gynecology,The Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin,Heilongjiang,150001,China;3 Department of Obstetrics and Gynecology,Qiqihar Traditional Chinese Medicine Hospital,Qiqihar,Heilongjiang,161000,China)ABSTRACT Objective:To study the effect of acupuncture at Baihui point on the behavioral changes and the expression ofN-methyl-D-aspartate receptor(NMDAR)-related proteins in the hippocampus of mice with postpartum depression.Methods:ThirtyC57BL/6 female mice were randomly divided into control group,model group and treatment group,30 mice in each group.The mice inthe model group and the treatment group were subcutaneously injected with dexamethasone sodium phosphate to establish a postpartumdepression mouse model during pregnancy,and the mice in the control group were subcutaneously injected with the same amount of nor-mal saline as a control.The mice in the treatment group were treated with acupuncture at Baihui point for 28 days after delivery,whilethe mice in the model group and control group were not treated.The 24-hour food consumption and body weight of the three groups ofmice,the time spent in the white box and the number of shuttles in the black and white box in the black and white box experiment,andthe immobility time in the forced swimming experiment and the immobility time in the tail suspension experiment were compared.At thesame time,the expression levels of NMDA receptors(NR2A and NR2B),cAMP-binding protein(CREB)and calmodulin kinase II(CaMKII)protein in the hippocampus of the three groups of mice were detected by immunoblotting.Results:Before treatment,the24-hour food consumption,body weight,white box residence time,black and white box shuttle times and CREB protein expression inhippocampus of postpartum depression mice were significantly lower than those of control mice(P0.05).The time of swimming in dif-ferent states,the time of tail suspension and the protein expression levels of NR2A,NR2B and cAMP in the hippocampus were significantly higher than those in the control group(P0.05).After treatment,the 24-hour food consumption,body weight,white box residence*基金项目：黑龙江省卫生健康委科研项目（2020-397）；齐齐哈尔市科技局计划项目（GSFGG-2021166）作者简介：刘晶（1984-），女，博士，副主任医师，研究方向：针刺治疗产后抑郁，E-mail：通讯作者：常萍（1973-），女，硕士，主任医师/教授，研究方向：针灸治疗妇产科疾病，E-mail：（收稿日期：2022-04-15 接受日期：2022-05-11）20现代生物医学进展Progress in Modern Biomedicine Vol.23NO.1JAN.2023time,number of black and white box shuttles,and CREB protein expression in hippocampus of the acupuncture Baihui point treatmentgroup were significantly higher than those of the model group(P0.05).However,the swimming time in different states,tail suspensionimmobility time and the expression levels of NR2A,NR2B and cAMP proteins in the hippocampus were significantly lower than those inthe model group(P0.05).Conclusion:Acupuncture at Baihui point can significantly improve the behavior of mice with postpartum de-pression and improve their exercise ability,which may be related to the expression of NMDAR-related proteins in mice with postpartumdepression.Key words:Baihui point;Acupuncture;Postpartum depression;NMDAR-related proteinChinese Library Classification（CLC）:R-33;R245;R714.6Document code:AArticle ID:1673-6273(2023)01-20-05前言产后抑郁症是产妇产褥期出现典型抑郁的发作，属产褥期精神综合征之一1,2。流行病学统计数据显示，在全球范围内产后抑郁患病率约为 1015%，我国研究报道的产后抑郁发病率约为 1.152.1%，并且初胎产后抑郁患者再次分娩产后抑郁的发生率高达 50%。尽管大部分产后抑郁患者在发病 3-6 月内可自行恢复，但严重的产后抑郁患者其临床症状可持续 1-2 年3-6。产后抑郁症患者常常感觉身体不适，虽然这是一种精神类疾病，不过大多人都会有躯体症状，比如便秘，食欲减退，消化不良，胸闷气短等，并且患者也常常会有自杀倾向7,8。此外，产后抑郁不仅会引起产妇身体不适，而且会影响新生儿的语言能力、智力发育以及身高体重7,8。因此，对产后抑郁患者因进行及时的干预治疗。近年来，多项研究表明9-12，针灸可有效改善抑郁患者临床症状。百会穴位于头顶正中线与两耳尖连线的交叉处，穴居颠顶，联系脑部，是调节大脑