棘胸蛙致病性荧光假单胞菌的分离鉴定徐悦玉1，林德2，赵淑芳1*（1.丽水学院医学院，浙江丽水323000；2.丽水学院附属第一医院检验科，浙江丽水323000）摘要：为进一步研究棘胸蛙烂皮病的致病菌，从丽水龙泉某养殖场挑选出患有烂皮病的棘胸蛙,并从病蛙患处分离出一株新致病菌。通过形态观察、革兰氏染色、质谱分析、16SrRNAPCR产物测序、人工感染试验和系统进化树分析，鉴定该菌株为荧光假单胞菌。纸片扩散法药敏实验结果表明：该菌株对链霉素、庆大霉素、阿米卡星、多黏菌素和氧氟沙星高度敏感，对氯霉素和复方新诺明中度敏感，对红霉素、头孢唑林、青霉素和氨苄西林产生耐药。关键词：棘胸蛙；荧光假单胞菌；药物敏感性doi：10.3969/j.issn.2095-3801.2023.02.003中图分类号：S947.2文献标志码：A文章编号：2095-3801（2023）02-0017-06IsolationandIdentificationofPathogenicPseudomonasFluorescensfromQuasipaaSpinosaXUYueyu1,LINDe2,ZHAOShufang1*（1.SchoolofMedicine,LishuiUniversity,Lishui323000，Zhejiang；2.ClinicaLaboratory，TheFirstAffiliatedHospitalofLishuiUniversity，Lishui323000，Zhijiang）Abstract：Inordertofurtherstudythepathogenicbacteriaofrottenskindisease,QuasipaaspinosaswithrottenskindiseasewereselectedfromafarminLongquan,Lishui,andanewpathogenicbacteriawasisolatedfromtheaffectedpartofthesickQuasipaaspinosa.Throughmorphologicalobservation,Gramstaining,massspectrometryanalysis,sequencingof16SrRNAPCRproducts,artificialinfectiontestandphylogenetictreeanalysis,thestrainwasidentifiedasPseudomonasfluorescens.Theresultsofdrugsensitivitytestbydiskdiffusionmethodshowedthatthestrainwashighlysensitivetostreptomycin,gentamicin,amikacin,polymyxinandofloxacin,moderatelysensitivetochloramphenicolandcompoundsulfamethoxazole,andresistanttoerythromycin,cefuroxime,penicillinandampicillin.Keywords：Quasipaaspinosa;Pseudomonasfluorescens;drugsensitivity收稿日期：2022-08-21；修回日期：2022-11-24基金项目:浙江省公益技术应用研究项目“生态农业之石蛙烂皮病治疗新对策”（LGN20C190005）作者简介：徐悦玉，女，浙江松阳人。*通信作者：赵淑芳，女，湖南平江人，副教授，博士。丽水学院学报JOURNALOFLISHUIUNIVERSITY第45卷第2期Vol.45No.22023年3月Mar.2023棘胸...
