拟蕈状芽孢杆菌JYZ-SD...作对水杉生长和耐盐性的影响_李振倩.pdf

第 59 卷 第 1 期2 0 2 3 年 1 月林业科学SCIENTIASILVAESINICAEVol.59,No.1Jan.,2 0 2 3doi:10.11707/j.1001-7488.LYKX20210213收稿日期:2021-03-23;修回日期:2022-11-06。基金项目:国家重点研发计划项目(2017YFD0600104);江苏高校优势学科建设工程项目(PAPD)。吴小芹为通讯作者。拟蕈状芽孢杆菌 JYZ-SD5 与菌根真菌互作对水杉生长和耐盐性的影响李振倩吴小芹孔维亮(南方现代林业协同创新中心南京林业大学林学院南京 210037)摘要:【目的】研究盐胁迫下拟蕈状芽孢杆菌(JYZ-SD5)和裂褶菌(Be)互作对水杉生长和耐盐性的影响,以探究其作用机制,为利用有益微生物提高木本植物耐盐性以及盐碱地生物修复利用提供参考依据。【方法】盐胁迫下采用盆栽试验探讨拟蕈状芽孢杆菌 JYZ-SD5 和裂褶菌 Be 对水杉相关生理代谢指标和耐盐性的影响。【结果】1)与对照相比,各接菌处理均提高了水杉的生物量和根系生长参数,其中双接种 JYZ-SD5+Be 效果优于单接种,双接种 处理的水杉幼苗株高和地径分别提高 65.47%和 63.46%,根长、根表面积、根体积、总根尖数和分枝数分别显著提高 1.05、1.51、2.2、1.88 和 2.31 倍(P0.05)。2)在盐胁迫下,与未接种对照相比,各接菌处理均有效缓解了盐胁迫对水杉叶片的损害,盐害症状减轻;单接种 JYZ-SD5 分别显著提高了水杉可溶性糖、可溶性蛋白和脯氨酸12.4%、62.58%和 38.99%(P0.05),单接种 Be 以及双接种处理分别显著提高水杉脯氨酸 122%和 35.64%(P0.05)。3)盐胁迫下,与未接种对照相比,各接菌处理均有效降低水杉 H2O2 含量、O-2产生速率、丙二醛含量和电解质渗漏,其中单接种 JYZ-SD5 分别显著降低 26.58%、9%、15.85%和 18.76%(P0.05),单接种 Be 分别降低8.91%、7.77%、8.54%和 22.09%,双接种 JYZ-SD5+Be 分别显著降低 15.58%、26.38%、53.66%和 30.39%(P0.05);同时各接菌处理均显著降低了水杉的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)活性。4)盐胁迫下,与未接种对照相比,各接菌处理提高了水杉茎和叶中的 K+含量,降低了 Na+含量,从而降低了 Na+/K+比,尤以单接种 Be 效果最好,分别显著降低水杉茎和叶 Na+/K+比 23.84%和 26.58%(P0.05)。【结论】根际促生细菌 JYZ-SD5 与外生菌根真菌 Be 可促进水杉生长,并可通过提高水杉植株水分吸收、渗透调节物质积累、维持细胞膜稳定性、降低膜脂质过氧化水平、活性氧积累及抗氧化酶活以及降低 Na+/K+比来缓解盐胁迫对水杉的损伤,提高水杉对盐胁迫的耐受性。关键词:拟蕈状芽孢杆菌 JYZ-SD5;裂褶菌 Be;水杉;盐胁迫;耐盐性中图分类号:S718.7文献标识码:A 文章编号:1001-7488(2023)01-0099-11Effects of Interaction between Bacillus paramycoides JYZ-SD5 and Mycorrhizal Fungi on Growth and Salt Tolerance of Metasequoia glyptostroboidesLi ZhenqianWu XiaoqinKong Weiliang(Co-Innovation Center for the Sustainable Forestry in Southern ChinaCollege of Forestry,Nanjing Forestry UniversityNanjing 210037)Abstract:【Objective】This study investigated the effects of the interaction between Bacillus paramycoides JYZ-SD5 and Schizophyllum commune Be on the growth and salt tolerance of Metasequoia glyptostroboides under salt stress,in order to explore the mechanism of action,and to provide a reference for the use of beneficial microorganisms to improve the salt tolerance of woody plants and the bioremediation of saline-alkali land.【Method】A pot experiment was conducted to investigate the effects of B.paramycoides JYZ-SD5 and S.commune Be on the physiological and metabolic indexes and salt tolerance of M.glyptostroboides under NaCl stress.【Result】1)Compared with control seedlings,the biomass and root growth parameters of M.glyptostroboides were improved by different inoculation treatments,and the effect of co-inoculation with JYZ-SD5+Be was better than single inoculation with JYZ-SD5 or Be.The height growth,diameter growth,root length,root surface area,root volume,root tips number and branches of M.glyptostroboides of co-inoculation with 林业科学59 卷JYZ-SD5+Be were significantly increased by 65.47%,63.46%,and 1.05,1.51,2.2,1.88,and 2.31 times,respectively(P 0.05).2)Under salt stress,compared with uninoculated control,all inoculation treatments effectively alleviated the damage of salt stress on M.glyptostroboides leaves and relieved the symptoms of salt damage.Single inoculation with JYZ-SD5 improved the soluble sugar,soluble protein and proline significantly by 12.4%,62.58%,and 38.99%,respectively(P 0.05),single inoculation with Be and co-inoculation with JYZ-SD5+Be significantly improved proline by 122%and 35.64%(P 0.05).3)Under salt stress,compared with uninoculated control,all inoculation treatments effectively reduced the H2O2 content,O-2generation rate,MDA content and electrolyte leakage,among which,single inoculation with JYZ-SD5 significantly reduced by 26.58%,9%,15.85%,and 18.76%(P 0.05),single inoculation with Be reduced by 8.91%,7.77%,8.54%,and 22.09%,co-inoculation with JYZ-SD5+Be significantly reduced by 15.58%,26.38%,53.66%and 30.39%(P 0.05),respectively.At the same time,all inoculation treatments significantly reduced the activity of SOD,POD,CAT,APX and GR.4)Under salt stress,compared with uninoculated control,all inoculation treatments improved K+content and reduced Na+content,and thus reduced Na+/K+ratio which was the highest in the single inoculation with Be and was significantly reduced by 23.84%and 26.58%in shoots and leaves of M.glyptostroboides(P 0.05),respectively.【Conclusion】This study showed that B.paramycoides JYZ-SD5 and S.commune Be can promote the growth of M.glyptostroboides,alleviate the damage of salt stress on M.glyptostroboides and improve the tolerance of M.glyptostroboides to salt stress by raising the plant water absorption and accumulation of osmotic regulation substances,maintaining the cell membrane stability,reducing the level of membrane lipid peroxidation and the accumulation of reactive oxygen species and antioxidant enzymes activity and reducing the Na+/K+ratios.Key words:Bacillus paramycoides JYZ-SD5;Schizophyllum commune Be;Metasequoia glyptostroboides;Salt stress;Salt tolerance土壤盐渍化是目前世界范围内面临的一种日趋严重的土壤退化状况。盐胁迫可诱导植物的渗透胁迫、氧化胁迫和离子毒害,主要干扰植物的新陈代谢过程,损 害 根 系 水 分 和 养 分 的 吸 收(Paul et al.,2014)。如何缓解或降低盐碱化对植物造成的损害,是有效利用植物修复日益增长的盐碱地的关键。目前,利