DOI:10.3969/j.issn.1003-0972.2023.02.015文章编号:1003-0972(2023)02-0249-06南召辛夷SRAP遗传多样性分析及指纹图谱的构建王岚1*,郭晓琴1,张宏2,李靖靖1(1.郑州工程技术学院化工食品学院,河南郑州450044;2.南召县辛夷产业办公室,河南南召474650)摘要:利用毛细管电泳技术,鉴定SRAP(Sequence-relatedamplifiedpolymorphisms)引物在34个南召辛夷种系中扩增条带的遗传多样性,聚类分析并构建DNA指纹图谱,为南召辛夷的种系鉴定和分子育种提供理论依据。结果表明:(1)5对SRAP引物共扩增出71条带,其中59条多态性的条带(83.1%),每对引物扩增出多态性位点为9~15条,平均为11.8条;(2)选用多态性高、鉴别能力强的2对引物构建了34份南召辛夷种系基于22个位点的DNA指纹图谱;(3)34份南召辛夷的遗传相似系数为0.58~0.97。UPGMA聚类结果显示,34个南召辛夷样品可分为6大类群,且与采摘地无直接关系。关键词:南召辛夷;SRAP;遗传多样性;指纹图谱;分子标记中图分类号:Q943.2文献标识码:A开放科学(资源服务)标识码(OSID):GeneticDiversityAnalysesandFingerprintsConstructionofNanzhaoMagnoliaeFlosUsingSRAPWANGLan1*,GUOXiaoqin1,ZHANGHong2,LIJingjing1(1.CollegeofChemicalandFoodEngineering,ZhengzhouInstituteofTechnology,Zhengzhou450044,China;2.NanzhaoCountyXinyiIndustrialOffice,Nanzhao474650,China)Abstract:Thecapillaryelectrophoresisisusedfordetectingthepolymorphismsamong34NanzhaoMagnoliaeFlos(MF)linesamplifiedbySRAPprimers.ThegeneticsimilaritycoefficientandclusteranalysisandDNAfin...