接收日期：2022-11-18接受日期：2022-12-14基金项目：厦门市科技计划项目(3502Z20214ZD4001、3502Z20199008)*通信作者。E-mail:601517900@qq.com利用流式细胞术鉴定68份三角梅基因组大小与染色体倍性陈宜木，周群，钟颖颖，张万旗*，李可威，林悦其(厦门市园林植物园，福建厦门361000)摘要：以国内外收集的54份三角梅(Bougainvillea)资源和14份实生种质为材料，选用番茄(Solanumlycopersicum)为内参，采用流式细胞术估测三角梅基因组大小，并以二倍体品种为对照，计算三角梅染色体倍性。结果表明：(1)所选用的内参与待检测样品的最高值能完全分开，没有重叠峰，峰型比较清晰集中，可以对三角梅基因组大小进行有效估测。(2)供试材料中有34份二倍体，基因组大小为2.48～2.86Gb；有28份三倍体，基因组大小为3.65～4.22Gb；有5份四倍体，基因组大小为4.98～5.12Gb；另外，‘大金边杨梅’为2X、4X、6X混倍体。利用流式细胞法鉴定三角梅染色体倍性可缩短鉴定时间，提高鉴定效率。关键词：三角梅；流式细胞术；基因组大小；染色体倍性；育种Doi:10.3969/j.issn.1009-7791.2022.06.001中图分类号：S685文献标识码：A文章编号：1009-7791(2022)06-0417-07GenomeSizeEstimationandPloidyIdentificationof68ServingsBougainvilleabyFlowCytometryCHENYi-mu,ZHOUQun,ZHONGYing-ying,ZHANGWan-qi*,LIKe-wei,LINYue-qi(XiamenBotanicalGarden,Xiamen361000,FujianChina)Abstract:Usingdiploidvarietytomatoastheinternalreference,thegenomesizesof54collectedgermplasmathomeandabroadand14seedlingsofBougainvillearesourceswereestimatedbyflowcytometry,andthechromosomeploidywascalculatedwithdiploidasthecontrol.Theresultsshowedthat:(1)thepeaksoftheselectedsamplesassayedwerecompletelyseparatedfromthatofthecontrolwithoutoverlapping,andthepeakshapewasclearandconcentrated,suggestingthattheestimationofBougainvilleagenomesizeandploidywaseffective;(2)amongthetestedmaterials,34germplasmwerediploidswithgenomesizesof2.48–2.86Gb,28germplasmweretriploidswithgenomesizesof3.65–4.22Gb,5germplasmweretetraploidwithgenomesizesof4.98–5.12Gb.Inaddition,aBougainvilleagermplasmresource‘BigPhnomPenhbayberry’wasidentifiedasamixoploidof2X,4Xand6X.Theusageofflowcytometryassaycouldshortenthetimewhileimprovetheefficienc...
