ICS65.020.30CCSB41团体标准T/SHZSAQS00134—2022绵羊FecB基因标记辅助选择育种技术规程2022-10-05发布2022-10-05实施石河子市质量标准化协会发布全国团体标准信息平台T/SHZSAQS00134—2022I目次前言................................................................................II1范围..............................................................................12规范性引用文件....................................................................13术语和定义........................................................................13.1多羔..............................................................................13.2FecB基因.........................................................................13.3标记辅助选择......................................................................13.4主效基因..........................................................................13.5限制性内切酶......................................................................23.6限制性片段长度多态性..............................................................24主要试剂配制......................................................................25主要仪器..........................................................................26FecB基因标记辅助选择育种方法......................................................26.1样本采集..........................................................................26.2基因组DNA提取....................................................................36.3基因组DNA质检....................................................................36.4PCR引物序列及引物配制............................................................36.5PCR扩增体系......................................................................36.6PCR扩增反应循环参数..............................................................36.7PCR产物的电泳检测................................................................36.8RFLP酶切分析.....................................................................36.9酶切产物电泳分析..............
