ICS11.220B41团体标准T/CVDA1—2022动物西尼罗病毒中和抗体检测技术DetectingtechniqueofneutralizationantibodyagainstWestNilevirusinanimals2022-07-19发布2022-07-25实施中国兽药协会发布T/CVDAT/CVDA1—2022目次前言................................................................................II1范围...............................................................................12规范性引用文件.....................................................................13缩略语.............................................................................14试剂和材料.........................................................................15器材和设备.........................................................................26动物血清的采集.....................................................................27PRNT实验操作.......................................................................27.1安全防护.......................................................................27.2材料准备.......................................................................27.2.1细胞......................................................................27.2.2待测血清..................................................................27.2.3检测用细胞板..............................................................27.3检测操作.......................................................................37.3.1血清的稀释................................................................37.3.2病毒与血清的中和..........................................................37.3.3接种细胞..................................................................37.3.4蚀斑计数..................................................................38结果观察与判定.....................................................................48.1观察顺序.......................................................................48.2蚀斑观察结果判定...............................................................48.2.1试验成立条件.........................................
