ICS67.120.20B40团体标准T/SHZSAQS015—2020实时荧光定量PCR法高效检测绵羊多胎Fecb基因SNP位点技术规程2020-12-26发布2020-12-31实施石河子市质量标准化协会发布全国团体标准信息平台T/SHZSAQS015—2020I目次前言...............................................................................III1范围..............................................................................12规范性引用文件....................................................................13术语和定义........................................................................13.1多胎..............................................................................13.2Fecb............................................................................13.3实时荧光定量PCR法................................................................14主要试剂配制........................................................................14.120mg/mL蛋白酶K..............................................................24.2柠檬酸钠（ACD）抗凝剂...........................................................24.31mol/LTris....................................................................24.40.5MEDTA(pH8.0)...............................................................24.5TE(pH8.0).......................................................................24.6PBS.............................................................................24.7DNA抽提缓冲液...................................................................25检测方法..........................................................................25.1样本采集........................................................................25.2DNA提取.........................................................................25.3DNA浓度和纯度测定...............................................................36引物设计..........................................................................37实时荧光定量PCR法检测............................................................37.1实时荧光定量PCR法反应体系................
