GBT 19438.2-2004 H5亚型禽流感病毒荧光RT-PCR检测方法.pdfVIP免费

ICS11.220B41GB/T19438.2—2004H5RT-PCRMethodofthereal-timeRT-PCRforthedetectionofAvianInfluenzaVirusSubtypeH52004-2-152004-2-15GB/T19438.2—2004IGB/T1.1—20001GB/T19438.2—2004H5RT-PCR1ØëÎÌóÐÝÎØë2ÙÞñÌïçìíèòïóîððìÎÌóÐÝÎ3ÎÌóÐÝΗݬÎÒßÜÒßÐÞÍÜÛÐÝ4TaqManØëë’ÚßÓÎí’ÌßÓÎßÏë’ÎÏë’í’ÎÏÐÝÎÐÝÎÐÝÎ55.1ÎÒßóîðéëûÜÛÐÝÙÞêêèîóîððòðï³±´ñÔ°ØéòîÐÞÍÙÞñÌïçìíèòìóîððìßïîïîïë³·²ïððððËñ³ÔGB/T19438.2—2004ØëÎÌóÐÝÎïß5.25.2.1ïîððð¹5.2.2PCR5.2.324-205.2.40.5L10L5L20L20L200L200L1000L5.2.55.2.65.2.766.1ïîïîïë³·²ïêðî¸Û°°»²¼±®ºïòë³Ô6.26.2.1íëïòð³ÔÐÞÍÛ°°»²¼±®º6.2.2îòð¹ïð³ÔÐÞÍïð³ÔÐÞÍÛ°°»²¼±®ºíððð®ñ³·²ïð³·²Û°°»²¼±®º6.2.3Û°°»²¼±®º6.3îèîì¸óéðí77.1ÙÞñÌïçìíèòïóîððìÝ7.21)GB/T19438.2—20047.2.1²ïòë³ÔÛ°°»²¼±®º²7.2.2êððµÔîððµÔîððµÔë­ìïîððð®ñ³·²ïë³·²7.2.3éòîòïïòë³ÔÛ°°»²¼±®ºìððÔóîðéòîòîëððÔ7.2.4ïîððð®ñ³·²ïë³·²Û°°»²¼±®ºêððµÔéëû7.2.5ìïîððð®ñ³·²ïð³·²Û°°»²¼±®º7.2.6ìððð®ñ³·²ïð­Û°°»²¼±®ºí³·²ÎÒß7.2.7ïïµÔÜÛÐÝÎÒßîððð®ñ³·²ë­ÎÒßî¸ÎÌóÐÝÎóéð7.3ß×ÊØëÎÌóÐÝÎîððð®ñ³·²ë­ÐÝβ²RT-PCRTaq15µL0.25µLðòîë²ÎÌóÐÝÎÐÝÎïëµÔ7.4ÐÝÎéòîòéÎÒßïðÔëðð®ñ³·²íð­7.5RT-PCRéòìÐÝÎÐÝÎÎÌóÐÝÎØëìîñíð³·²çîñí³·²çîñïð­ìëñíð­éîñï³·²ëçîñïð­êðñíð­ìð88.1GB/T19438.2—20048.28.2.1ݬ8.2.2ݬîèòð8.38.3.1ݬ8.3.2ݬíðòð8.3.3ݬíðòðݬGB/T19438.2—20041AH5RT-PCRA.148tests/íð³ÔïØëÎÌóÐÝÎéëðÔïÎÌóÐÝÎÐÝÎïñïîëËñÔïîÔïÜÛÐÝï³Ôïï³ÔïÎÒßï³ÔïA.2A.2.1ÎÒßìA.2.2ÜÛÐÝïûÜÛÐÝÎÒßA.2.3ÎÌóÐÝÎA.3A.3.1A.3.2A.3.3ÎÌóÐÝÎÎÌóÐÝÎA.3.4óîðê