菊花珍品梨香菊离体快繁技术研究邵会会(唐山园林科学研究所,河北唐山063000)摘要以梨香菊的幼嫩叶片为外植体,MS为基本培养基,研究了不同植物生长调节剂及基本培养基对其愈伤组织诱导、增殖、分化及丛生芽增殖和生根的影响,旨在建立离体快繁体系。结果表明:用MS+6-BA2.0mg/L+NAA0.4mg/L作为培养基可以较快地建立间接再生体系,诱导愈伤组织;愈伤组织增殖的最佳培养基为MS+6-BA0.5mg/L+NAA1.0mg/L;MS+6-BA2.0mg/L+NAA0.05mg/L为最佳的分化培养基,分化的芽数较多,芽体健壮饱满;MS+6-BA1.0mg/L最适合丛生芽增殖,增殖系数高,芽体粗壮、质量好;1/2MS+NAA1.0mg/L培养基中梨香菊生根最好。关键词梨香菊;组培;增殖;分化中图分类号S682.1文献标识码A文章编号0517-6611(2023)11-0070-03doi:10.3969/j.issn.0517-6611.2023.11.016开放科学(资源服务)标识码(OSID):StudyontheTechniqueofinvitroRapidPropagationofChrysanthemumcuriosaLixiangjuSHAOHui-hui(GardenInstituteofTangshan,Tangshan,Hebei063000)AbstractTheleavesofLixiangjuwereusedasexplant,plantgrowthregulatorsandbasicmediumswerestudiedoncallusinduction,multi-plication,differentiationandshootmultiplication,rootingandgrowth.TheresultsshowedthatuseMS+6-BA2.0mg/L+NAA0.4mg/Lascul-turemediumtoestablishindirectregenerationsystemandinducecallusmorefasterandbetter.MS+6-BA0.5mg/L+NAA1.0mg/Lwasthebestculturemediumforcallusmultiplication.MS+6-BA2.0mg/L+NAA0.05mg/Lwasthebestculturemediumforcallusdifferentiation,thenumberofbudswasmorethansomeothertreats,budsstrongerandfuller.Thevigorofbudswasbest.WhenusedMS+6-BA1.0mg/Lascul-turemediumforshootmultiplicationandgrowth,multiplicationcoefficienthigher,thelengthofbudslongersignificantlythanothertreats.Thebestrootingmediumwas1/2MS+NAA1.0mg/L.KeywordsLixiangju;Tissueculture;Multiplication;Differentiation基金项目河北省建设科技研究计划项目。作者简介邵会会(1985—),女,河北沧州人,工程师,硕士,从事园林植物育种与栽培养护研究。收稿日期2022-07-29梨香菊又名白梨香,花色洁白、素雅,因具有酷似鸭梨的香味而得名。轻轻晃动花头就有酷似鸭梨的香气扑鼻而来,更有“盈手生香”的说法,中花期前香味较浓,后随花朵绽放程度的提高香味逐渐变淡,栽培历史达数百年,是目前中国数千个菊花品种中唯一一个具...
