金盏菊枯萎病病原鉴定及防治药剂筛选.pdfVIP免费

http：//bnxb・cbpt.cnki.netdoi：10.13473/j.cnki.issn.1002-3186.2023.0207北京农学院学报2023,38(2)：38-43JournalofBeijingUniversityofAgriculture金盏菊枯萎病病原鉴定及防治药剂筛选唐冉，郭孜雪，陈莹莹，隆林芳，陈艳*（北京农学院生物与资源环境学院/农村农业部华北都市农业重点实验室，北京102206）摘要：【目的】为了明确金盏菊枯萎病的病原菌种类、筛选防治药剂。【方法】对分离得到的金盏菊枯萎病病原菌进行了形态学和分子生物学鉴定，基于该病原菌的EF-la序列构建了系统发育树并对其致病性进行了测定。【结果】结果表明，引起金盏菊枯萎病的病原为尖抱镰刀菌刃twn通过生长速率法和盆栽法筛选了防治金盏菊枯萎病的药剂，结果表明，30%噁霉灵水剂和80%乙蒜素乳油对供试菌株F.oxysporum菌丝生长的抑制活性最强，同时对于该类病原菌的盆栽防效最好，高达8&09%和82.14%0【结论】研究结果为金盏菊枯萎病病原菌的鉴定及其田间防治提供了参考。关键词：金盏菊；枯萎病；病原鉴定；药剂防治中图分类号：S432.1文章编号：1002-3186（2023）02-0038-06文献标志码：APathogenicidentificationandcontrolofwiltdiseaseonCalendulaofficinalisTANGRan,GUOZixue,CHENYingying,LONGLinfang.CHENYan*(KeyLaboratoryforNorthernUrbanAgricultureofAgricultureandRuralAffairs9CollegeofBioscienceandResourcesEnvironment,BeijingUniversityofAgriculture,Beijing102206,China)Abstract:CalendulaofficinalisL.),animportantmedicinalandornamentalplant,isgrownwidelyinChina.Inre­centyears>wholeplantwiltingphenomenonwasoftenobservedincalendula,whichcausedlargeeconomiclosses・[Objective]Toidentifythepathogenicspeciesofcalendulawiltandscreenthecontrolagents5thetypicalwiltsampleswerecollectedinthisexperiment,andthediseasedsampleswereisolatedandpurifiedtoobtainthreefungalstrains.QMethods]Morphological,mo­lecularandpathogenicityassayswereperformedforthesepathogens?andthephylogenetictreewasconstructedbasedonEF-lasequences.[Results]TheresultsindicatedthattheetiologicalagentcausingCalendulawiltdiseasewasFusariumoxysporum.Thecontrolagentsagainstwiltwerescreenedbygrowthrateandpottingmethods,andtheresultsshowedthat30%oxamycinprimateand80%allicincreamwereeffectiveagainstthetestedstrainF.oxys...