农业环境科学学报JournalofAgro⁃EnvironmentScience2023，42（6）:1257-12662023年6月郭琳琳，刘盼，王晶晶，等.纳米塑料与盐胁迫对菠菜种子和幼苗的毒性效应[J].农业环境科学学报,2023,42（6）：1257-1266.GUOLL,LIUP,WANGJJ,etal.ToxicityofthecombinationofnanoplasticsandsaltstressonseedsandseedlingsofSpinaciaoleraceaL.[J].JournalofAgro-EnvironmentScience,2023,42（6）：1257-1266.纳米塑料与盐胁迫对菠菜种子和幼苗的毒性效应郭琳琳1，刘盼1，王晶晶2，谌柄旭3（1.沧州师范学院生命科学系，河北沧州061001；2.大连大学生命健康学院，辽宁大连116622；3.沧州环创环保技术服务有限公司，河北沧州061001）ToxicityofthecombinationofnanoplasticsandsaltstressonseedsandseedlingsofSpinaciaoleraceaL.GUOLinlin1,LIUPan1,WANGJingjing2,CHENBingxu3（1.DepartmentofLifeScience,CangzhouNormalUniversity,Cangzhou061001,China;2.CollegeofLifeandHealth,DalianUniversity,Dalian116622,China;3.CangzhouHuanchuangEnvironmentalProtectionTechnologyServiceCo.,LTD.,Cangzhou061001,China）Abstract：Thisstudyaimedtounderstandtheimpactofnanoplasticsandsaltstressonvegetablecrops.Specifically,weinvestigatedtheeffectsofpolystyrenenanoplastics（PSNPs）（200,400,800mg·L-1,and1600mg·L-1）withaparticlesizeof100nmandalsothecombinatorialeffectofPSNPsandNaCl（50,100,150mmol·L-1,and200mmol·L-1）ontheseedgerminationandseedlinggrowthofspinach（SpinaciaoleraceaL.）.Wenoticedthat,incomparisontothecontrol,singlePSNPstreatments（>200mg·L-1）significantlyreducedthegerminationrate,vigor,andindexofspinachseeds.Further,superoxidedismutase（SOD）activitywasdecreased,andthesolubleproteincontentwassignificantlyincreasedundercultivationwithPSNPs（>400mg·L-1）.Moreover,lowconcentrationsofPSNPspromotedperoxidase（POD）activityandincreasedchlorophyllcontent,whilehighPSNPsconcentrationsalsoinducedaninhibitoryeffectonPODandchlorophyll.ComparedwithNaClalone,treatmentswithlow（200mg·L-1）orhigh（800mg·L-1）concentrationsofPSNPsandNaCl（PSNPs+NaCl）reducedgerminationrate,vigor,andindexofspinachseeds,andaggravatedtheinhibitoryeffectsofNaCl.Thecombined收稿日期...
