研究报告ResearchReport百脉根WRKY基因家族生物信息学分析马培杰李亚娇舒健虹王小利*贵州省农业科学院草业研究所,贵阳,550006*通信作者,398189177@qq.com摘要WRKY转录因子是一个超家族的调节因子，参与植物的多种生物过程和逆境胁迫反应。然而，在百脉根中对WRKY家族还没有相关的研究。本研究共鉴定出79个LjWRKYs基因，它们不均匀分布在各染色体上的基因位点编码，与拟南芥AtWRKYs进行系统发育分析，分为I、II和III类。保守基序和外显子-内含子数目表明79个LjWRKYs在不同亚簇中均具有较大的差异，顺式作用元件结果显示在上游2000bp启动子区域包含多种逆境胁迫、激素和生长发育相关的元件。共线性结果有36对片段复制和1对串联重复复制共线性基因，且Ka/Ks均小于1。本研究通过分析百脉根WRKY基因家族的基本结构与性质，为深入研究百脉根WRKY基因家族提供了基础。关键词百脉根;WRKY;基因家族BioinformaticsAnalysisofWRKYGeneFamilyintheLotuscorniculatusMaPeijieLiYajiaoShuJianhongWangXiaoli*InstituteofPrataculture,GuizhouAcademyofAgriculturalSciences,Guiyang,550006*Correspondingauthor,398189177@qq.comDOI:10.13271/j.mpb.021.004606AbstractWRKYtranscriptionfactorsareasuperfamilyofregulatoryfactorsinvolvedinavarietyofplantbiologi-calprocessesandstressresponses.However,thereisnoresearchonWRKYfamilyinLotuscorniculatusL..Atotalof79LjWRKYsgeneswereidentifiedinthisstudy.Theywereencodedbylociunevenlydistributedoneachchro-mosome.PhylogeneticanalysiswasconductedwithAtWRKysinArabidopsisthaliana,andtheyweredividedintoI,IIandIIIcategories.Theresultsofconservedmotifsandexonintronnumbersshowedthat79LjWRKYsweredif-ferentindifferentsubclusters.Cisactingelementsshowedthattheupstream2000bppromoterregioncontainedavarietyofstress,hormoneandgrowthrelatedelements.Theresultsshowedthattherewere36pairsoffragmentreplicationand1pairoftandemrepeats,andtheKa/Kswerelessthan1.Inthisstudy,thebasicstructureandprop-ertiesofWRKYgenefamilywereanalyzed,whichprovidedthebasisforfurtherstudyofWRKYgenefamily.KeywordsLotuscorniculatus;WRKY;Genefamily基金项目：本研究由贵州省重点基金项目(黔科合基础〔2019〕1449号)、贵州省农业科学院青年基金项目(黔农科院青年基金〔2019〕23号)、贵州省创...
