大肠杆菌脂多糖对大鼠牙囊干细胞生物学行为的影响姜震颜燕宏韩雪蒋备战*同济大学口腔医学院·同济大学附属口腔医院儿童口腔科,上海牙组织修复与再生工程技术研究中心上海200072[摘要]目的:探究大肠杆菌脂多糖(E.coliLPS)对大鼠牙囊干细胞(rDFSCs)生物学行为的影响。方法:体外培养鉴定rDFSCs,使用不同浓度(0、1、10μg/mL)E.coliLPS处理细胞,通过qRT-PCR检测炎症因子的表达;CCK-8法检测细胞增殖情况;划痕实验和Transwell实验检测细胞迁移能力;碱性磷酸酶(ALP)染色和茜素红染色分别评估rDFSCs的矿化情况,并利用qRT-PCR检测其成骨相关基因表达水平。结果:相比于对照组,LPS组IL-6、IL-1β及TNF-α的mRNA表达水平升高(P<0.05);1μg/mLLPS促进rDFSCs增殖及迁移能力(P<0.05),而10μg/mLLPS对其增殖、迁移影响不明显;LPS处理后ALP染色降低、矿化结节数量减少,且成骨标志物RUNX2、COL1及OPN表达下降(P<0.05)。结论:E.coliLPS模拟的炎症微环境下,rDFSCs成骨分化能力受到明显抑制,低浓度(1μg/mL)LPS刺激可促进rDFSCs的增殖和迁移。[关键词]脂多糖;牙囊干细胞;细胞增殖;细胞迁移;成骨分化[文献标识码]A[文章编号]1671—7651(2023)06—0534—07[doi]10.13701/j.cnki.kqyxyj.2023.06.013EffectsofEscherichiaColiLipopolysaccharideonBiologicalBehaviorsofRatDentalFollicleStemCells.JIANGZhen,YANYanhong,HANXue,JIANGBeizhan*.DepartmentofPediatrics,SchoolandHospitalofSto-matology,TongjiUniversity,ShanghaiEngineeringResearchCenterofToothRestorationandRegeneration,Shanghai200072,China.[Abstract]Objective:ToinvestigatetheeffectsofEscherichiacolilipopolysaccharide(E.coliLPS)onbiologicalbehaviorsofratdentalfolliclestemcells(rDFSCs).Methods:rDFSCswereculturedandidentified,andthenweretreatedwithdifferentconcentrationsofE.coliLPS(0,1,10μg/mL).ThemRNAlevelsofpro-inflammatorygenesweremeasuredbyquantitativereal-timefluorescencepolymerasechainreaction(qRT-PCR).TheeffectsofLPSonproliferationandmigrationabilityofrDFSCswereassessedbyCCK-8andscratchtestplustranswellassay,respectively.TheosteogeniccapabilityofrDFSCswasdetectedbyalkalinephosphatase(ALP)stainingandalizarinredstaining,andtheirosteogenic-relatedgenemRNAlevelsweremeasuredbyqRT-PCR.Results:Thegeneex-pressionsofIL-6,IL-1β,andTNF-...
