安徽农学通报2023年05期动植物·微生物·食用菌·中药材黑木耳黑色素的液体发酵培养及提取工艺研究王小漫柴新义于士军向玉勇孙星（滁州学院生物与食品工程学院，安徽滁州239000）摘要为提高黑木耳黑色素的产量，以碱溶酸沉法对黑木耳液体发酵菌丝体中的黑色素进行提取，以黑色素得率为指标，采用单因素试验和正交设计试验，对黑木耳黑色素的液体发酵生产及提取工艺条件进行了研究。结果表明，黑木耳黑色素的最优液体发酵生产工艺条件为起始pH5.5、25℃、培养9d；最佳提取工艺条件为NaOH浓度2mol/L、料液比1∶40、浸提1.5h。黑木耳黑色素得率可达1.55%。研究结果可为黑木耳工业化液体发酵生产黑色素提供重要的参考。关键词黑木耳黑色素；液体发酵；黑色素得率；工艺条件优化中图分类号Q939.96文献标识号A文章编号1007-7731（2023）05-0031-06StudyonLiquidFermentationCultureandExtractionTechnologyofMelaninfromAuriculariaauriculataWANGXiaomanCHAIXinyiYUShijuanXIANGYuyongSUNXing（SchoolofBiologyandFoodEngineering,ChuzhouUniversity,ChuzhouAnhui239000）AbstractInordertoimprovetheyieldofAuriculariaauriculamelanin,themelanininliquidfermentationmyceliumofAuriculariaauriculawasextractedbyalkalisolutionandacidprecipitation.Takingtheyieldofmelaninastheindex,theliquidfermentationproductionandextractionconditionsofAuriculariaauriculamelaninwerestudiedbysinglefactortestandorthogonaldesigntest.TheresultsshowedthattheoptimumliquidfermentationconditionsofAuriculariaauriculamelaninwereasfollows:initialpH5.5,25℃andculturefor9days;TheoptimumextractionconditionswereNaOHconcentrationof2mol/L,solid-liquidratioof1∶40andextractionfor1.5h.TheyieldofmelaninfromAuriculariaauriculacanreach1.55%.TheresultscanprovideanimportantreferencefortheindustrialliquidfermentationofAuriculariaauriculatoproducemelanin.KeywordsAuriculariaauriculamelanin;liquidfermentation;melaninyield;optimizationofprocessconditions黑木耳（Auriculariaauricula）隶属担子菌亚门（Basidiomycotina）、层菌纲（Hymenomycetes）、木耳目（Auriculariales）、木耳科（Auriculariaceae）、木耳属（Auricularia），在我国云南、东北、河南等多地广泛分布[1]。黑木耳中富含蛋白质、多糖、氨基酸、黑色素、黄酮、多...
