蝉科昆虫病原真菌物种多样性（英文）_林俨.pdf

Research paper 研究论文 22 March 2023,42(3):663-676 菌物学报 Mycosystema ISSN1672-6472 CN11-5180/Q Doi:10.13346/j.mycosystema.220185 This work was supported by the Plan for Outstanding Young Talents in Colleges and Universities of Anhui Province(gxyqZD2019013)and the Postgraduate Innovation Fund Project of Anhui Agricultural University(2020ysj-21).*Corresponding author.E-mail: ORCID:LIN Yan(0000-0002-8797-1816)Received:2022-05-24;Accepted:2022-07-07 Copyright 2023 Institute of Microbiology,CAS.All rights reserved.| Http:/journals- Tel:+86-10-64807521 菌物学报 663 蝉科昆虫病原真菌物种多样性 林俨1,2，汪婷1，张胜利3，陈名君1*1 安徽农业大学 微生物防治重点实验室，安徽 合肥 230036 2 新罗区自然资源局 新罗区林业技术推广中心，福建 龙岩 364000 3 淮南师范学院生物工程学院，安徽 淮南 232001 摘 要：蝉科昆虫在自然界具有重要作用，是果树等林木的刺吸性害虫，同时蝉与真菌组成的虫菌共生体也具有重要经济价值。近十年来，通过对我国昆虫病原真菌资源的调查，共采集获得 102 株蝉科昆虫罹患病原真菌标本，并保存于安徽农业大学昆虫病原真菌研究中心(RCEF)。本研究从蝉科罹患病原真菌标本中分离得到 81 株菌株，并对其多样性进行了研究。基于不同培养基的形态特征观察并结合 ITS、SSU、LSU、TEF、RPB1 和 RPB2 基因构建系统发育树，发现寄生蝉科的菌株属于 3 科 6 属 15 种。本研究重点描述了一个中国新记录种高水紫孢菌；此外，一些类棒束孢属菌株被修订为蝙蝠蛾萨姆森菌；除上述物种外，还鉴定出球孢白僵菌、绿色绿僵菌、蝉花虫草、柱孢绿僵菌、环链虫草、玫烟色虫草、澳洲白僵菌、布氏白僵菌、细脚虫草、棕色绿僵菌和长座细虫草。其中球孢白僵菌、蝉花虫草和绿色绿僵菌是优势种，多度达总数的 58%。而其他 12 个物种分离频率较低。关键词：蝉科；昆虫病原真菌；系统发育分析；分类学；物种多样性 引用本文 林俨，汪婷，张胜利，陈名君，2023.蝉科昆虫病原真菌物种多样性.菌物学报，42(3):663-676 Lin Y,Wang T,Zhang SL,Chen MJ,2023.Species diversity of the entomopathogenic fungi that parasitize the Cicadidae insects.Mycosystema,42(3):663-676 LIN Yan et al./Species diversity of the entomopathogenic fungi that parasitize the Cicadidae insects 研究论文 菌物学报 664 Species diversity of the entomopathogenic fungi that parasitize the Cicadidae insects LIN Yan1,2,WANG Ting1,ZHANG Shengli3,CHEN Mingjun1*1 Provincial Key Laboratory of Microbial Control,Anhui Agricultural University,Hefei 230036,Anhui,China 2 Xinluo Forestry Technology Extension Center,Xinluo Natural Resources Bureau,Longyan 364000,Fujian,China 3 School of Biological Engineering,Huainan Normal University,Huainan 232001,Anhui,China Abstract:The insects of family Cicadidae,stinging pests of trees such as fruit trees,are of important injurious orchard pests but their symbionts of fungi and cicadas become ecomoically valuable.Our surveys of entomopathogenic fungi in China over recent decades have gathered 102 samples of cicada cadavers which are deposited in the Anhui Provincial Key Laboratory of Microbial Control of Anhui Agricultural University.In this study,81 fungal cultures were successfully isolated from these cadavers of Cicadidae.Observation of morphological characteristics of the isolates on various media and phylogenetic analysis based on partial sequence of ITS,SSU,LSU,TEF,RPB1 and RPB2 were carried out.Experiment results show that these strains parasitizing the Cicadidae belong to 15 species of 6 genera in 3 families.A new Chinese record species,Purpureocillium takamizusanense isolated from adult cicadas,was reported and some Isaria-like strains were referred to Samsoniella hepiali.Chinese known species such as Beauveria bassiana,Metarhizium viridulum,Cordyceps chanhua,M.cylindrosporum,C.cateinannulata,C.fumosorosea,B.australis,B.brongniartii,C.tenuipes,M.brunneum and Ophiocordyceps longissima were also identified.Among them B.bassiana,C.chanhua and M.viridulum were dominant,which account for 58%of the total,and the rest twelve species were found to be rare.Keywords:Cicadidae;entomopathogenic fungi;phylogenetic analysis;taxonomy;species diversity Cicadas usually live in temperate to tropical forests around the world,with a long underground juvenile period and a brief aboveground adult life(Shrestha et al.2017).They are well known for the loud calls of males(Young&Bennet-Clark 1995).Although Cicadidae are not major agricultural and forestry pests,they are potentially harmful to many fruit trees and even forests(Mehdipour et al.2016).Comparing to the nymphal cicadas,adult cicadas require more leg strength and flexibility for feed on xylem sap of host plants and live on the trunk of a tree canopy(Wang 2021).Some fungi can infect and kill adult cicadas.Massospora cicadina Peck specifically attacks the adults of periodical cicadas(Cooley et al.2018),and the spores usually remain dormant in the soil during the non-outbreak periods.Both cicadas and Ophiocordyceps fungi are the most diversified in warm and humid tropical/subtropical regions in the world(Matsuura et al.2018).In Asia,people use the cicada/fungus complex for traditional medicinal purposes(Zhu et al.1998;Holliday&Cleaver 2008).Some cicadicolous fungi such as Cordyceps chanhua,are regarded as medicinal mushrooms in oriental society.Because of this magical life process,they are also vividly called“chanhua”.They were recorded in the ancient Chinese classics“Bencao Tujing”(1061)and“Compendium of Materia Medica”(1590),and were used as medicinal materials by ancient Chinese people.Thus far,over 3 000 species of cicadas and nearly 500 species of Ophiocordyceps-allied entomopathogenic fungi have been described(Sanborn 2014).However,the taxonomy and systematics of fungi-cicada groups are Research paper 22 March 2023,42(3):