第36卷第1期2023年3月Vol.36No.1Mar.2023海南师范大学学报（自然科学版）JournalofHainanNormalUniversity（NaturalScience）二维液相色谱法同时测定婴幼儿配方奶粉中的维生素A、D、E李莲1,2,3，黄柳霞1,2,3，罗雪婷1,2,3（1.广东省食品工业公共实验室，广东广州511442；2.广东省食品工业研究所有限公司，广东广州511442；3.广东省质量监督食品检验站，广东广州511442）摘要：本文建立了二维液相色谱法同时测定婴幼儿配方奶粉中维生素A、E、D含量。样品经皂化、萃取、浓缩、定容，经二维反相色谱法测定，对所建立的方法进行方法学验证，结果表明：维生素A、D、E标准曲线线性良好，R2>0.999，维生素A、E、D检出限分别为8、0.65、30μg/100g；定量限分别为25、2、100μg/100g，加标回收率为87.3%~96.5%，RSD为0.45%~3.80%，同时与国标法进行比较，该方法更加简便快捷，灵敏度高，结果也相对较准。关键词：二维液相色谱法；维生素A；维生素D；维生素E；婴幼儿配方奶粉中图分类号：O657.72;TS252.51文献标志码：A文章编号：1674-4942（2023）01-0046-07SimultaneousDeterminationofVitaminA,DandEinPowderedInfantFormulabyTwo-dimensionalLiquidChromatographyLILian1,2,3，HUANGLiuxia1,2,3，LUOXueting1,2,3（1.GuangdongProvincialPublicLaboratoryofFoodIndustry，Guangzhou511442，China；2.GuangdongFoodIndustryInstituteCo.Ltd.，Guangzhou511442，China；3.GuangdongQualitySupervisionfoodInspectionStation，Guangzhou511442，China）Abstract:Inthispaper,two-dimensionalliquidchromatographywasestablishedtosimultaneouslydeterminethecontentofvitaminsA,EandDinpowderedinfantformula.Thesamplesweresaponified,extracted,concentrated,volumed,and⁃determinedbytwo-dimensionalreversed-phasechromatography,andtheestablishedmethodwasverifiedmethodological⁃ly,andtheresultsshowedthatthestandardcurvesofvitaminA,DandEwerelinearwithR2>0.999,thedetectionlimitsofvitaminsA,EandDwere8,0.65,30μg/100g.Thequantitativelimitswere25,2,100μg/100g,therecoveryrateofspikedwere87.3%~96.5%,andtheRSDwere0.45%~3.8%.Comparedwiththenationalstandardmethod,themethodwassimplerandfaster,withhighsensitivityandrelativelyaccurateresults.Keywords:two-dimensionalliquidchromatography;vitaminA;vitaminD;vitaminE;infan...
