广东化工2023年第6期·170·www.gdchem.com第50卷总第488期枸杞多糖含量测定的方法比较余永英1，刘建欣1，张伟1，王平1，高小林1，杨元元1，俱雪妮1，张丹2*(1．汉中天然谷生物科技股份有限公司，陕西汉中723001；2．陕西理工大学化学与环境科学学院，陕西汉中723001)[摘要]目的：优化枸杞多糖含量测定的最佳方法，为枸杞提取物多糖含量测定方法标准化提供依据。本文采用三种测定方法分别测定枸杞提取物中多糖的含量并比较不同方法的差异。方法：参照《中华药典》采用紫外可见分光光度法测定、苯酚-硫酸分光光度法和以葡聚糖为参照的苯酚-硫酸分光光度法。结果：药典法测得的多糖含量为2.93%;苯酚-硫酸分光光度法测得的多糖含量为2.63%;以葡聚糖为参照的苯酚-硫酸分光光度法测得的多糖含量均值为1.29%。3种不同测定方法测定枸杞的多糖含量存在较大的差异。结论：苯酚硫酸法测得的结果为药典法的0.9倍，是葡聚糖分光光度法的2倍。通过比较3种方法的差异性，可根据实际样品及实际需求，选择最佳检测方法。[关键词]枸杞子；多糖；含量测定；分光光度法[中图分类号]TQ[文献标识码]A[文章编号]1007-1865(2023)06-0170-04ComparisonofMethodsforDeterminationofLyciumBarbarumPolysaccharideYuYongying1,LiuJianxin1,ZhangWei1,WangPing1,GaoXiaolin1,YangYuanyuan1,JuXueni1,ZhangDan2*(1.HanzhongTianranguBiologicalTechnologyGroupLimitedCompany,Hanzhong723001；2.ShaanxiKeyLaboratoryofCatalysis,CollegeofChemistryandEnvironmentScience,ShaanxiUniversityofTechnology,Hanzhong723001,China)Abstract:Objective:ThebestmethodfordeterminationofpolysaccharidecontentinLyciumbarbarumwillbeoptimizedtoprovideabasisforthestandardizationofdeterminationmethodforpolysaccharidecontentinLyciumbarbarumextract.Inthispaper,threedeterminationmethodswereusedtodeterminethecontentofpolysaccharidesinLyciumbarbarumextractandcomparethedifferences.Methods:Accordingto“ChinesePharmacopoeia”,UV-visiblespectrophotometry,phenol-sulfuricacidspectrophotometryandphenol-sulfuricacidspectrophotometrywithdextranasreferencewereused.Results:Thepolysaccharidecontentmeasuredbypharmacopoeiamethodwas2.93%;thepolysaccharidecontentmeasuredbyphenol-sulfuricacidspectrophotometrywas2.63%;theaveragepolysaccharidecontentmea...
