第43卷第3期高师理科学刊Vol.43No.32023年3月JournalofScienceofTeachers′CollegeandUniversityMar.2023文章编号:1007-9831(2023)03-0060-06不同生长类型番茄幼苗形态比较及差异表达lncRNAs筛选梁琦航1,王安琪1,刘嘉仪1,杨静雯1,张珍珠2,付学鹏1(1.齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006;2.黑龙江农业工程职业学院生物制药分院,黑龙江哈尔滨150025)摘要:以4种不同生长类型的番茄为研究对象,比较了它们幼苗的形态,筛选了茎尖组织中差异表达的lncRNAs.结果表明,无限生长型的番茄在幼苗期生长快,节间较长,而有限生长型的番茄幼苗则生长慢.Micro-Tom番茄株高最矮,最高时约为8cm;最高的为无限生长型的吉农大桃,东升红和齐研矮粉在幼苗期的株高相差不大.筛选到不同生长类型幼苗茎尖差异表达的lncRNAs42个,这些lncRNAs将会为番茄不同株型的调控机制研究提供参考.关键词:番茄;生长类型;形态学;lncRNA中图分类号:S18文献标识码:Adoi:10.3969/j.issn.1007-9831.2023.03.013MorphologicalcomparisonandscreeningofdifferentialexpressionoflncRNAsindifferentgrowthtypetomatoseedlingsLIANGQihang1,WANGAnqi1,LIUJiayi1,YANGJingwen1,ZHANGZhenzhu2,FUXuepeng1(1.SchoolofLifeSciences,AgricultureandForestry,QiqiharUniversity,Qiqihar161006,China;2.BiologicalPhamacyAcademy,HeilongjiangAgriculturalEngineeringVocationalCollege,Harbin150025,China)Abstract:Fourdifferentgrowthtypesoftomatowereselectedasresearchobjects,andtheirseedlingmorphologywascompared.LncRNAsdifferentiallyexpressedinstemapextissuesoftomatoseedlingswerescreened.Theresultsshowedthattheinfinitegrowthtypeoftomatogrewfastattheseedlingstagewithlongerinternodes,whilethelimitedgrowthtypeoftomatoseedlingsgrewslowly.Micro-Tomtomatoplantheightwasthelowest,thehighestwasabout8cm.ThehighestseedlingwasJinongdataowithinfinitegrowth,whileDongshenghongandQiyanaifenhadlittledifferenceinplantheightatseedlingstage.Forty-twolncRNAsdifferentiallyexpressedinstemapexofseedlingsofdifferentgrowthtypeswerescreened,andtheselncRNAswillprovidereferencesforthestudyofregulatorymechanismsofdifferenttomatoplanttypes.Keywords:tomato;growthtype;morphology;l...