6种植物精油体外抗肿瘤活性研究张辉1,徐端妙1*,林秀君1,陈莉娟1,刘浩凯2(1.景宁畲族自治县生态林业发展中心,浙江景宁323500;2.景宁县畲族自治县自然资源和规划局,浙江景宁323500)摘要:为了探究6种植物精油的抗肿瘤活性,选择6种柏科植物———日本花柏(Chamaecyparispisifera)、日本扁柏(C.obtusa)、细叶花柏(C.pisiferaPlumoso)、台湾扁柏(C.obtusavar.formosana)、罗汉柏(Thujopsisdolabrata)和日本冷杉(Abiesfirma)作为原料,采用水蒸气蒸馏技术提取植物精油,以植物精油作为油相,以吐温80为表面活性剂、乙醇为助表面活性剂制备O/W型微乳体系。采用MTT比色法检测6种精油对HepG2、A549和U937等3株肿瘤细胞株的增殖抑制作用。实验结果显示:6种精油对HepG2、A549和U937肿瘤细胞株的增殖均显示抑制作用,其中日本扁柏叶精油抑制3株肿瘤细胞的增殖作用比较明显。因此,6种植物精油具有较强的抗肿瘤活性,具有潜在的开发应用价值。关键词:植物精油;抗肿瘤;微乳体系doi:10.3969/j.issn.2095-3801.2023.02.007中图分类号:S763.306.4文献标志码:A文章编号:2095-3801(2023)02-0043-05OnAntitumorActivityofSixPlantEssentialOilsinVitroZHANGHui1,XUDuanmiao1*,LINXiujun1,CHENLijuan1,LIUHaokai2(1.EcologicalForestryDevelopmentCenterofJingningSheAutonomousCounty,Jingning323500,Zhejiang;2.NaturalResourcesandPlanningBureauofJingningSheAutonomousCounty,Jingning3235000,Zhejiang)Abstract:Toinvestigateanti-tumoractivityoftheessentialoilsfrom6plants,sixplantsincludingChamaecyparispisifera.C.obtusa.C.pisiferaPlumoso.C.obtusavar.Formosanahadbeenchosen.ThujopsisdolabrataandA-biesfirmawereusedasrawmaterials,essentialoilwasextractedbysteamdistillation.TheO/Wmicroemulsionsystemwaspreparedwithplantessentialoilasoilphase,Tween80assurfactantandethanolascosurfactant.MTTassaywasusedtoinvestigatetheinhibitoryeffectofessentialoilsontheproliferationofHepG2,A549andU937cellliness.TheresultsshowedthatthesixessentialoilshadinhibitoryeffectsontheproliferationofHepG2,A549andU937tumorcelllines,amongwhichtheessentialoilfromC.obtusahadabetterinhibitoryeffectonthethreecellssignificantly.Therefore,6kindsofplantessentialoilshaveanti-tumoractivityandpoten...