山羊ESR1·TENM1和...性及其与繁殖性状相关性研究_李昆谕.pdf

山羊 ES1TENM1 和 THEM4 多态性及其与繁殖性状相关性研究李昆谕1，2，刘玉芳2，陶 林3，江炎庭1，欧阳依娜1，储明星3*，洪琼花1(1云南省畜牧兽医科学院，云南昆明 650224;2河北工程大学生命科学与食品工程学院，河北邯郸 056001;3中国农业科学院北京畜牧兽医研究所/农业农村部动物遗传育种与繁殖重点实验室，北京 100193)摘要 目的 分析 ES1、TENM1 和 THEM4 单核苷酸多态性(SNPs)与山羊繁殖性状的相关性。方法 采用 MassAAYSNP 分型技术对 ES1、TENM1 和 THEM4 SNPs 位点进行分型，检测其在云上黑山羊、济宁青山羊和辽宁绒山羊 3 个群体中的遗传学特征，并对其多态性与山羊繁殖性状(产羔数、初生窝重、断奶窝重)进行相关性分析。结果 群体遗传学分析结果表明，ES1 g76097125CT 位点在济宁青山羊和辽宁绒山羊中为低度多态(PIC025)，在云上黑山羊中为中度多态(025PIC050);ES1 g76139051AG 在云上黑山羊、济宁青山羊和辽宁绒山羊中为中度多态(025PIC050)。TENM1 g17333655CT 和 g17372494AG 位点在 3 个山羊群体中表现为低度多态(PIC025);TENM1 g17236451GC 和 g17586866TC 位点在 3 个山羊群体中的多态性(PIC)为 005 036。THEM4g101275605GA 位点在 3 个山羊群体中为低度多态(PIC025)。卡方检验结果表明，ES1 g76139051 AG、THEM4 g101275605GA位点在云上黑山羊、济宁青山羊和辽宁绒山羊中处于 HardyWeinberg 平衡状态(P005)。相关分析结果表明，ES1 g76097125CT 位点 CT 和 TT 型山羊的初生窝重显著高于 CC 型(P005);TENM1 g17236451GC 位点 GC 型山羊的产羔数和断奶窝重显著高于 GG 型(P005)，g17333655CT 位点 CC 型山羊产羔数显著高于 CT 型(P005);其余位点多态性与云上黑山羊产羔数、初生窝重和断奶窝重无显著相关(P005)。结论TENM1 g17236451GC 位点可作为云上黑山羊产羔数和断奶窝重选择的潜在分子标记;TENM1g17333655CT 位点可作为云上黑山羊产羔数选择的潜在分子标记;ES1 g76097125CT 位点可作为云上黑山羊初生窝重选择的潜在分子标记。该研究结果可为山羊分子育种和标记辅助选择提供理论基础。关键词山羊;SNPs;繁殖性状;ES1;TENM1;THEM4中图分类号S827文献标识码A文章编号05176611(2023)01007906doi:103969/jissn05176611202301017开放科学(资源服务)标识码(OSID):Study on the Polymorphism of ES1，TENM1 and THEM4 and Their Correlation with the eproductive Traits of GoatsLI Kun-yu1，2，LIU Yu-fang2，TAO Lin3et al(1Yunnan Academy of Animal Husbandry and Veterinary Sciences，Kunming，Yunnan650224;2School of Life Sciences and Food Engineering，Hebei University of Engineering，Handan，Hebei 056001;3Key Laboratory of AnimalGenetics，Breeding and eproduction of Ministry of Agriculture and ural Affairs/Institute of Animal Sciences，Chinese Academy of Agricultur-al Sciences，Beijing 100193)Abstract Objective To analyze correlation between single nucleotide polymorphisms(SNPs)of ES1，TENM1 and THEM4 and the repro-ductive traits of goats Method SNPs loci of ES1，TENM1 and THEM4 were typed by using MassAAY SNP typing technology，and theirgenetic characteristics in three populations(Yunshang Black Goat，Jining Grey Goat and Liaoning Cashmere Goat)were detectedThe correla-tion between their polymorphism and the reproductive traits(litter size，litter weight at birth，litter weight at weaning)of goats were analyzed esult The population genetic results showed that ES1 g76097125CT locus was low polymorphic(PIC025)in Jining Grey Goat and Li-aoning Cashmere Goat，and it was moderately polymorphic(025PIC050)in Yunshang Black GoatES1 g76139051AG was moderatelypolymorphic(025PIC050)in Yunshang Black Goat，Jining Grey Goat and Liaoning Cashmere GoatTENM1 g17333655CT and g17372494AG loci were low polymorphic(PIC025)in the three goat populationsThe polymorphism of TENM1 g17236451GC andg17586866TC loci was 005036 in the three goat populationsTHEM4 g101275605GA locus was low polymorphic(PIC025)in thethree goat populationsThe chi-square test showed that ES1 g76139051AG and THEM4 g101275605GA loci were in Hardy-Weinbergequilibrium(P005)in Yunshang Black Goat，Jining Grey Goat and Liaoning Cashmere GoatThe results of correlation analysis showed thatthe litter weight at birth of the goats with CT and TT genotypes in ES1 g76097125CT locus was significantly higher than that with CC geno-type(P005)The litter size and litter weight at weaning of the goats with GC genotype in TENM1 g17236451GC locus were significantlyhigher than those with GG genotype(P005)，the litter size of the goats with CC genotype in g17333655CT locus was significantly higherthan that with CT genotype(P005)The polymorphism of the other loci had no significant correlation with the litter size，litter weight at birth，litter weight at weaningin Yunshang Black Goat(P005)Conclusion TENM1 g17236451GC locus could be used as a potential molecu-lar marker for selecting the litter size and litter weight at weaning in Yunshang Black GoatTENM1 g17333655CT locus could be used as apotential molecular marker for the selection of the litter size in Yunshang Black GoatES1 g76097125CT locus could be used as a potentialmolecular marker for selecting the litter weight at birth in Yunshang Black GoatThe results of this study could provide the theoretical basis forthe molecular breeding and marker-assisted selection in goatsKey wordsGoat;SNPs;eproductive traits;ES1;TENM1;THEM4基金项目国家现代农业产业技术体系资助项目(CAS38);云南省重大科技专项计划项目(202102AE090039);云南省万人计划 产业技术领军人才项目;中国农业科学院科技创新工程项目(CAASZDW202106，ASTIPIAS13);河北省自然科学基金青年项目(C2019402293)。作者简介李昆谕(1996)，男，甘肃定西人，硕士研究生，研究方向:动物遗传育种。*通信作者，研究员，博士，博士生导师，从事羊优异繁殖性状分子机理研究。收稿日期20211129产羔性状对于山羊的生产尤其重要，而提高单胎产羔数是提高产羔性状最主要的途径。山羊产羔数是一个极其复杂的性状，且产羔性状遗传力较低(约为 01)，因此如何快速提高山羊产羔数是养羊业一个亟待解决的难题。与传统育种手段相比，分子遗传育种具有准确度高、可操作性强、育种成本低等优点，能更加高效、快速地对山羊产羔性状进行改良。利用分子标记技术有利于高繁殖力山羊的筛选，从而加快育种进程，可为山羊的选育工作提供更多便利1。ES1(estrogen receptor 1)基因定位于 9 号染色体，拥有 12 个外显安徽农业科学，JAnhui AgricSci 2023，51(1):7984子。雌激素受体 由 ES1 编码，通过与靶基因上的特异性效应元件结合，从而调节靶基因的表达2。雌激素受体 为雌激素的受体，与雌激素结合后，对胚胎、乳腺和雌性繁殖周期中卵泡的生长发育都发挥着重要作用3。研究表明，ES基因是调控猪高产仔数的主效基因之一4。Tao 等5 通过GWAS、OH 分析和选择特征检测发现 ES1 是参与山羊卵巢功能的一个重要候选基因。TENM1 和 THEM4 基因