Chem.J.ChineseUniversities,2023,44(2),2022060220220602(1/7)CHEMICALJOURNALOFCHINESEUNIVERSITIES高等学校化学学报研究论文铝纳米粉末活化树突状细胞的作用朱歌1，李知涵2，刘堃2，孙天盟1（1.吉林大学第一医院,长春130000；2.吉林大学化学学院,超分子结构与材料国家重点实验室,长春130012）摘要采用电爆炸法制备金属铝纳米粉末(ALEX),验证了其免疫增强效应及肿瘤预防效果.首先,利用铝金属丝电爆炸法,并应用羧基端基聚合物配体反应的方法合成了分散性良好的ALEX.利用静电物理吸附将抗原卵清蛋白(OVA)、抗原多肽(Peptide)接枝于ALEX表面,傅里叶变换红外光谱检测证明抗原与ALEX成功覆合.其次,验证了ALEX增强特异性免疫反应的效果.通过体外实验证明,ALEX可提高树突状细胞(DCs)的抗原提呈效果;同时,对免疫后小鼠血清抗体进行了定量检测,结果表明ALEX可显著提高小鼠体内抗原特异性抗体的水平.最后,利用小鼠黑色素瘤模型,验证了含有ALEX的肿瘤疫苗能够有效预防肿瘤的发生.关键词铝纳米粉末；电爆炸法；树突状细胞活化；肿瘤疫苗中图分类号O614；O631文献标志码Adoi：10.7503/cjcu20220602EffectofAluminiumNanopowdersonActivationofDendriticCellsZHUGe1，LIZhihan2，LIUKun2，SUNTianmeng1*（1.TheFirstHospital，JilinUniversity，Changchun130000，China；2.StateKeyLaboratoryofSupramolecularStructureandMaterials，CollegeofChemistry，JilinUniversity，Changchun130012，China）AbstractAluminumnanopowders（ALEX）werepreparedbyelectricalexplosionandtheireffectsofimmuneen⁃hancingandtumorpreventionwereverified.TheALEXwithwell-dispersionweresynthesizedbyelectricalexplosionofwiresmethod，combingwithcarboxylgrouppolymerligandreaction.Theantigenicproteinovalbumin（OVA）andpeptidewasgraftedontoALEXsurfacebyphysicalelectrostaticadsorption.FouriertransforminfraredspectroscopydemonstratedthegraftingofantigentoALEX.TheimmuneenhancingeffectofALEXwasverified：invitroexperimentsshowedthatALEXcouldimprovetheantigenpresentationfunctionofdendriticcellsandthequantitativedetectionofantibodiesintheserumofmiceafterimmunizationshowedthatALEXcouldsignificantlyincreasethetiterofantigen-specificantibodies.Finally，thetumorvaccinecontainingALEXwasprovedtobeeffectivefortumorpreventionusingm...
