临床医学研究与实践2023年3月第8卷第7期研究原著Analysisofcharacteristicsofshort-chainfattyacidsinfecesofpatientswithcolorectalcancerZHANGBin,WENBo,LIUZhenggang,CHENDairu,YUYuanyou,LISheng*(ShaoyangHospitalAffiliatedtoUniversityofSouthChina,Shaoyang422000,China)ABSTRACT:ObjectiveToanalyzethecharacteristicsofshort-chainfattyacids(SCFAs)infecesofpatientswithcolorectalcancer(CRC),andprovideusefulinformationforearlyscreeningandtreatmentofthedisease.MethodsFromJanuarytoJuly2022,44CRCpatients(20casesofrightcoloncancer,24casesofleftcoloncancer),42casesofcolonadenomatouspolyps(CAP)admittedingastrointestinalsurgerydepartmentand40healthypeopleinphysicalexaminationcenterofShaoyangHospitalAffiliatedtoUniversityofSouthChinawereselectedanddividedintoCRCgroup,CAPgroupandHPgroup.TheconcentrationofSCFAsinfeceswasdeterminedbygaschromatography-massspectrometry(GC-MS).TheGC-MSdetectionresultswasrecord;theconcentrationofSCFAsinfecesofthethreegroupswascompared;theconcentrationofSCFAsinfecesofpatientswithrightcoloncancerandleftcoloncancerwascompared;partialleastsquaresdiscriminantanalysis(PLS-DA)wasusedtodeterminethecharacteristicsofSCFAsinfecesofeachgroup.ResultsSixSCFAshadgoodlinearrelationshipwithintheirrespectivelinearrange(R2≥0.9998).ComparedwiththeHPgroup,theconcentrationofaceticacid,propionicacid,butyricacidandtotalSCFAsinfecesoftheCRCgroupdecreased,whiletheconcentrationofisovalericacidincreased,andthedifferenceswerestatisticallysignificant(P<0.05);comparedwiththeCAPgroup,theconcentrationofbutyricacidinfecesoftheCRCgroupdecreased,andthedifferencewasstatisticallysignificant(P<0.05).Therewerenosignificantdifferencesintheconcentrationsofaceticacid,propionicacid,isobutyricacid,butyricacid,isovalericacid,valericacidandtotalSCFAsinfecesbetweentheCAPgroupandtheHPgroup(P>0.05).TheconcentrationofaceticacidandtotalSCFAsinfecesofpatientswithrightcoloncancerwashigherthanthatofpatientswithleftcoloncancer,andthedifferenceswerestatisticallysignificant(P<0.05).ThePLS-DAmodelshowedthattheseparatio...
