菌根真菌在改善附生兰干旱耐...：以禾叶贝母兰为例（英文）_刘娜.pdf

Research paper 研究论文 22 January 2023,42(1):217-230 菌物学报 Mycosystema ISSN1672-6472 CN11-5180/Q Doi:10.13346/j.mycosystema.220471 资助项目：国家自然科学基金(32170013)；中国医学科学院医学与健康科技创新工程-重大协同创新项目(2021-I2M-1-031)This work was supported by the National Natural Sciences Foundation of China(32170013)and the CAMS Initiative for Innovative Medicine(2021-I2M-1-031).*Corresponding author.E-mail: ORCID:XING Xiaoke(0000-0002-1845-5076)Received:2022-11-14;Accepted:2022-11-18 Copyright 2023 Institute of Microbiology,CAS.All rights reserved.| Http:/journals- Tel:+86-10-64807521 菌物学报 217 邢晓科 中国医学科学院药用植物研究所研究员、博士生导师。加拿大不列颠哥伦比亚大学(UBC)、比利时鲁汶大学(KU Leuven)访问学者。研究方向为菌根生态学，近年来在菌根真菌与兰科植物的共存分化、地理分布、生态位形成、菌根互作网络结构及菌根生态功能等方面进行了系列研究。菌根真菌在改善附生兰干旱耐受性中的作用：以禾叶贝母兰为例 刘娜，赵泽宇，石利欣，杨露娜，邢晓科*中国医学科学院北京协和医学院 药用植物研究所，北京 100193 摘 要：干旱胁迫是在多种生态系统中影响植物生存、发育及产量的最主要的非生物因素之一。菌根共生已被证明可以提高植物对干旱的耐受性。兰科植物对菌根真菌有非常高的依赖性，但是有关兰科菌根真菌是否可以提高宿主植物的耐旱性以及能提高到什么程度还少有报道。在本研究中，我们检测了一株分离自附生型兰科植物禾叶贝母兰 Coelogyne viscosa 的胶膜菌属真菌 Tullasnella sp.hy-111 对宿主植物幼苗生长及耐旱性的影响，并从转录组水平检测了该菌根真菌对禾叶贝母兰幼苗基因表达的影响。结果显示，接种hy-111不仅能显著提高幼苗的生物量、与耐旱相关的酶活性以及渗透调节物质的富集，而且还能显著诱导植物抗性途径相关基因的上调表达。本研究表明菌根真菌能改善生长于胁迫的附生生境中的兰科植物对于干旱的耐受性，并可能在兰科植物的生态适应中起到重要作用。关键词：兰科菌根；干旱胁迫；生态功能；转录组 引用本文 刘娜，赵泽宇，石利欣，杨露娜，邢晓科，2023.菌根真菌在改善附生兰干旱耐受性中的作用：以禾叶贝母兰为例.菌物学报，42(1):217-230 Liu N,Zhao ZY,Shi LX,Yang LN,Xing XK,2023.The role of mycorrhizal fungi in improving drought tolerance of epiphytic orchid:a case study on Coelogyne viscosa.Mycosystema,42(1):217-230 LIU Na et al./The role of mycorrhizal fungi in improving drought tolerance of epiphytic orchid:a case study.研究论文 菌物学报 218 The role of mycorrhizal fungi in improving drought tolerance of epiphytic orchid:a case study on Coelogyne viscosa LIU Na,ZHAO Zeyu,SHI Lixin,YANG Luna,XING Xiaoke*Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China Abstract:Drought stress is one of the most important abiotic factors that affects plant survival,development and yield in many ecosystems.Mycorrhizal symbiosis has been proven to enhance drought resistance of host plants.Orchids are well known for their strong reliance on mycorrhizal fungi,but it remains unclear whether and to what extent mycorrhizal symbiosis affects drought tolerance in orchids.In this research,the effects of Tullasnella sp.hy-111 isolated from the roots of epiphytic orchid Coelogyne viscosa on the growth and drought tolerance of orchid seedlings were tested,and differences in gene expression at the transcriptome level were examined.The results showed that inoculation of hy-111 significantly enhanced biomass of seedlings and increased the activities of drought resistance related enzymes and the accumulation of osmoregulatory substances.hy-111 inoculum also significantly induced up-regulating expression of genes involved in the plant resistance pathways.These results suggest that orchid mycorrhizal fungi are capable of improving drought tolerance of orchids growing in stressful epiphytic habitats and probably play an important role in the ecological adaptation of epiphytic orchids.Keywords:orchid mycorrhiza;drought stress;ecological function;transcriptomics Mycorrhiza is the most ubiquitous symbiotic association between plants and fungi in almost all ecosystems(Brundrett 2009).Since first named by Frank in 1885,mycorrhizae have been shown to facilitate the acquisition of essential nutrients from the soil,while plants generally transfer photosynthetically fixed carbon to their fungal partners(Bonfante&Genre 2010;van der Heijden et al.2015).Additionally,mycorrhizal symbiosis is essential for plants to resist both biotic and abiotic stresses(Ruiz-Snchez et al.2010).Drought stress is considered to be one of the most important abiotic factors negatively affecting plant survival,development and yield in many ecosystems(Kramer&Boyer 1995).The symptoms of drought caused by limited water availability in plants include wilting of the plants and decrease in photosynthetic activity,stomatal conductance,water use efficiency and total chlorophyll content(Zoghi et al.2019).In addition,drought stress also impairs the electron transport system,leading to the formation of activated oxygen(Saraswathi&Paliwal 2011).In order to avoid drought stress or to increase its tolerance of drought,plants have undergone a series of changes at morphological,anatomical and cellular levels(Ruiz-Lozano 2003;Seleiman et al.2021).Although these morphological and anatomical changes can be of vital importance for plants to cope with drought stress,increasing evidence published in recent years suggests that rhizosphere microbes,including mycorrhizal fungi play an important role in increasing plant tolerance to drought stress(Rillig et al.2019).Orchid mycorrhizal fungi(OMF)play an important role in the life cycle of orchids.Germination of orchids seeds relies on mycorrhizal fungi because their tiny seeds are devoid of endosperm.Most adult orchids retain mycorrhizal associations as well,but their role is less