2022年第12期摘要：以兰州百合多糖为原料，采用β-甘露聚糖酶进行酶解，通过单因素试验与L9（33）正交试验研究底物质量分数、酶添加量、酶解时间对还原糖生成量的影响，优化得出最佳工艺条件；同时以维C为对照，运用DPPH、ABTS自由基、羟自由基和超氧自由基试验来评价兰州百合多糖酶解产物的抗氧化活性。结果表明，兰州百合多糖的最佳酶解工艺为底物质量分数0.2%，酶添加量0.5%，酶解时间4h，在此条件下还原糖的生成量为0.8062±0.0084mg/mL。所得酶解产物对4种自由基的清除能力在试验浓度范围内均强于未经酶解兰州百合多糖的清除能力，且呈一定的质量浓度依赖关系。当质量浓度为4mg/mL时，兰州百合多糖对羟自由基的清除率为69.16%，酶解后产物对羟自由基的清除率达92.18%，接近于阳性对照维C。酶解提高了兰州百合多糖的抗氧化活性。关键词：兰州百合；多糖；酶解工艺；抗氧化活性中图分类号：TQ461；R284.2文献标志码：Adoi：10.16693/j.cnki.1671-9646（X）.2022.12.036StudyontheEnzymaticHydrolysisofLanzhouLilyPolysaccharideanditsAntioxidantActivityinVitroHUIHeping1，WANGPing1，ZHANGXinchi2，CAIWei1，LIXiaodong3，LIGuannan1，LIUYingnan1（1.GansuVocationalCollegeofAgriculture，Lanzhou，Gansu730020，China；2.SchoolofPharmacy，GansuUniversityofTraditionalChineseMedicine，Lanzhou，Gansu730010，China；3.GansuHospitalofTraditionalChineseMedicine，Lanzhou，Gansu730050，China）Abstract：UsingLanzhoulilypolysaccharideasrawmaterial，itwashydrolyzedbyβ-mannanase.Theeffectsofsubstratecon-centration，enzymedosageandhydrolysistimeontheyieldofreducingsugarwerestudiedbysinglefactortestandL9（33）or-thogonaltest，andtheoptimaltechnologicalconditionwasobtained.Meanwhile，theDPPH，ABTSfreeradical，hydroxylradicalandsuperoxideradicalexperimentswereconductedtoevaluatetheantioxidantactivityoftheenzymatichydrolysisprod-uctsofLanzhoulilypolysaccharide，withVCasthecontrol.Theresultsshowedthattheoptimalenzymatichydrolysisconditionwassubstrateconcentration0.2%，enzymedosage0.5%，andenzymatictime4h.Undertheseconditions，theyieldofreduc-ingsugarwas0.8062±0.0084mg/mL.Thescavengingabilityoftheobtainedenzymatichydrolysisproductstothefourfreeradicalswasstrongertha...
