《食品工业》2023年第44卷第2期28工艺技术基于响应面法的花豇豆酯酶提取工艺马月琼，陈守慧*，魏新林*上海交通大学农业与生物学院，上海食品安全工程技术研究中心（上海200240）摘要基于酶抑制原理建立果蔬中农药残留的快速检测方法,以固蓝B盐作为显色剂,通过比较面粉、麦麸和13种植物种子的总酯酶活力和比活力,确定用于农药残留检测的最佳植物酯酶源。结果表明,花豇豆酯酶的总酯酶活力和比活力最大,确定花豇豆为最佳植物酶源。结合单因素试验、响应面试验和验证试验确定植物酯酶最佳提取条件:料液比1︰5(g/mL)、提取液pH8、浸提时间2h、离心速率8000r/min,此最佳提取条件下酶活力为9.081U/mL。因此,在该条件下能够较好地从花豇豆中提取植物酯酶,并且能够保持较高的酶活力。关键词植物酯酶;筛选;提取优化;响应面分析ExtractionTechnologyofCowpeaEsteraseBasedonResponseSurfaceMethodologyMAYueqiong,CHENShouhui*,WEIXinlin*SchoolofAgricultureandBiology,ShanghaiJiaoTongUniversity,ShanghaiFoodSafetyEngineeringTechnologyResearchCenter(Shanghai200240)AbstractBasedontheprincipleofenzymeinhibition,arapiddetectionmethodforpesticideresiduesinfruitsandvegetableswasestablished.UsingfastblueBsaltasacolordevelopingagent,bycomparingthetotalesteraseactivityandspecificactivityofflour,wheatbranand13plantseeds,thebestplantesterasesourceforpesticideresiduedetectionwasdetermined.Theresultsshowedthatthetotalesteraseactivityandspecificactivityofcowpeaesterasewerethelargest,andcowpeawasdeterminedasthebestplantenzymesource.Combinedwithsinglefactorexperiment,responsesurfaceexperimentandverificationexperiment,theoptimumextractionconditionsofplantesteraseweredeterminedasfollows:theratioofmaterialtoliquidwas1︰5(g/mL),thepHoftheextractwas8,theextractiontimewas2h,andthecentrifugalratewas8000r/min.Underthisoptimumextractioncondition,theenzymeactivitywas9.081U/mL.Therefore,undertheseconditions,plantesterasescouldbebetterextractedfromcowpea,andhighenzymeactivitycouldbemaintained.Keywordsplantesterase;screening;extractionoptimization;responsesurfaceanalysis联合国预测，到2050年全球人口可达到92亿。随着人口密度的增加，食物的需求也在不断地增加。因此，对于提高作物...
