有机化学ChineseJournalofOrganicChemistryARTICLE*Correspondingauthors.E-mail:lijinghua@henu.edu.cn;luowen83@163.comReceivedJune11,2022;revisedAugust10,2022;publishedonlineSeptember15,2022.ProjectsupportedbytheKeyScienceandTechnologyProgramofHenanProvince(Nos.212102311030,222102310671)andtheFoundationofHenanEdu-cationalCommittee(No.22A350012).河南省科技攻关(Nos.212102311030,222102310671)和河南省教育厅基金(No.22A350012)资助项目.178http://sioc-journal.cn/©2023ShanghaiInstituteofOrganicChemistry,ChineseAcademyofSciencesChin.J.Org.Chem.2023,43,178～185DOI:10.6023/cjoc202206016研究论文基于黄酮的长波长荧光探针用于检测体外和体内生物硫醇周思仪a丁旭a赵永梅b李景华*,a罗稳*,a(a河南大学天然药物与免疫工程重点实验室河南开封475004)(b河南应用技术职业学院制药工程学院河南开封475004)摘要半胱氨酸(Cys)、同型半胱氨酸(Hcy)和谷胱甘肽(GSH)等生物硫醇在生理和病理过程中起着至关重要的作用,尤其是GSH在肿瘤细胞中过度表达,可以作为肿瘤的生物标志物.然而,同时区分活的正常细胞和肿瘤细胞存在着困难和挑战,因此设计并合成了一种基于N,N-二甲氨基萘黄酮(3)的肉眼可见“关-开”型长波长荧光探针(4).该探针对生物硫醇具有较高的选择性和较低的检测限,机理研究表明,生物硫醇催化探针的酯键断裂,生成了强荧光的黄酮3.该探针可用于活细胞和小鼠体内生物硫醇成像,并且对正常肝细胞HL-7702和肝癌细胞HepG2进行选择性成像.关键词长波长;荧光探针;黄酮;生物硫醇AFlavone-BasedLong-WavelengthFluorescentProbetoDetectBiothiolsinvitroandinvivoZhou,SiyiaDing,XuaZhao,YongmeibLi,Jinghua*,aLuo,Wen*,a(aKeyLaboratoryofNaturalMedicineandImmuno-Engineering,HenanUniversity,Kaifeng,Henan475004)(bPharmaceuticalEngineeringDepartment,HenanTechnicalInstitute,Kaifeng,Henan475004)AbstractBiothiols,suchascysteine(Cys),homocysteine(Hcy),andglutathione(GSH)playcrucialrolesinphysiologicalandpathologicalprocesses.GSH,especially,isoverexpressedinnumeroustypesoftumorcellsandcouldbeconsideredasabiomarker.However,owingtothedifficultyandchallengeinsensingnormalandcancercellssimultaneously,itisimportanttosolvethisprobleminbiologicalprocesses.Herein,anaked-eyevisible,“off-on”lo...
