《食品工业》2023年第44卷第2期283分析检测基于离子色谱法测定乳及乳制品中亚硝酸盐含量张丽芳，张淼怡，刘宇奇，郑百芹，周璇，谷守国*1.唐山市食品药品综合检验检测中心（唐山063000）；2.河北省农产品质量安全检测工程技术创新中心（唐山063000）；3.唐山市功能性农产品产业技术研究院（唐山063000）摘要建立离子色谱法测定乳及乳制品中亚硝酸盐含量。样品以乙腈为蛋白沉淀剂,3.6mmol/LNa2CO3为淋洗液,流速为0.7mL/min,经MetrosepASuup7-250/4.0色谱柱分离,电导检测器分析,外标法定量。结果表明,亚硝酸盐出峰时间14.4min,在0.01~2.0μg/mL质量浓度内线性关系良好,检出限为0.02~0.04mg/kg,定量限为0.04~0.10mg/kg,方法的平均回收率为85.4%~101.3%,相对标准偏差为1.5%~7.1%(n=6)。试验方法简单高效、重复性好、准确度高,可用于乳与乳制品中亚硝酸盐含量的快速定量分析。关键词离子色谱法;亚硝酸盐;乳及乳制品;定量检测DeterminationofNitriteinMilkandDairyProductsbyIonChromatographyZHANGLifang,ZHANGMiaoyi,LIUYuqi,ZHENGBaiqin,ZHOUXuan,GUShouguo*1.TangshanFoodandDrugComprehensiveTestingCenter(Tangshan063000);2.HebeiAgriculturalProductsQualityandSafetytestingInnovationCenter(Tangshan063000);3.TangshanAgriculturalProductQualityandSafetyTestingCenters(Tangshan063000)AbstractAmethodforthedeterminationofnitriteinmilkanddairyproductsbyionchromatographywasestablished.Theproteininthesamplewasprecipitatedbyacetonitrile.Theeluentwascomposedof3.6mmol/LNa2CO3,andtheflowratewas0.7mL/min.TheanalysiswasperformedonMetrosepASuup7-250/4.0separationcolumn,andtheconductivitydetectorwasusedfordetection.Theresultsshowedthatthepeaktimeofnitritewas14.4min.Thestandardcalibrationcurvewaslinearfornitriteintheconcentrationrangeof0.01-2.0μg/mL,thelimitofdetectionswere0.02-0.04mg/kg,andthelimitofquantificationswere0.04-0.1mg/kg.Therecoveriesofnitritefromsamplesspikedatthreedifferentconcentrationswerewithin85.4%to101.3%,andtherelativestandarddeviationwaswithin1.5%to7.1%(n=6).Thismethodwassimple,efficient,reproducibleandaccurate.Itcouldbeusedforrapidquantitativeanalysisofnitritecontentinmilkanddairyproducts.Keywordsionchromatography;nitrite;milkanddairyproducts;qua...
