基于Wnt信号通路探究上调...网膜病变模型大鼠的干预效果_郑丽.pdf

Metab，2020;31:956-68，e5.9Yi ZJ，Deng M，Scott MJ，et al Immune-responsive gene 1/itaconateactivates nuclear factor erythroid 2-related factor 2 in hepatocytes toprotect against liver ischemia-reperfusion injuryJ Hepatology，2020;72:1394-411.10Coll M，Perea L，Boon，et al Generation of hepatic stellate cellsfrom human pluripotent stem cells enables in Vitro modeling of liverfibrosis J Cell Stem Cell，2018;23(1):101-13，e711Tsuchida T，Friedman SL Mechanisms of hepatic stellate cell activa-tion J Nat ev Gastroenterol Hepatol，2017;14:397-411.12Friedman SL Hepatic stellate cells:protean，multifunctional，and e-nigmatic cells of the liver J Physiol ev，2008;88(1):125-72.13Yuan B，Huang HF，Qu SM，et al Gastrodin pretreatment protectsliver against ischemia-reperfusion injury via activation of the Nrf2/HO-1 pathway J Am J Chin Med，2020;48:1159-78.14Prieto I，Monsalve M OS homeostasis，a key determinant in liver is-chemic-preconditioning J edox Biol，2017;12:1020-5.15Bystrom P，Foley N，Toledo-Pereyra L，et al Ischemic precondition-ing modulates OS to confer protection in liver ischemia and reperfu-sion J Excli J，2017;16:483-96.16Luangmonkong T，Suriguga S，Mutsaers HAM，et al Targeting oxida-tive stress for the treatment of liver fibrosis J ev Physiol BiochemPharmacol，2018;175:71-102.17Bedossa P，Paradis V Transforming growth factor-beta(TGF-beta):a key-role in liver fibrogenesis J J Hepatol，1995;22:37-42.18Zhang Y，Feng XH，Derynck Smad3 and Smad4 cooperate with c-Jun/c-Fos to mediate TGF-beta-induced transcriptionJ Nature，1998;394:909-13.19Heldin CH，Miyazono K，ten Dijke P TGF-beta signalling from cellmembrane to nucleus through SMAD proteinsJ Nature，1997;390:465-71.2022-02-20 修回(编辑李和旭)基于 Wnt 信号通路探究上调 mi-184 对糖尿病视网膜病变模型大鼠的干预效果郑丽秦学维王利民姚贤凤陈梅伍丹丹(贵州中医药大学第一附属医院眼科，贵州贵阳550001)摘要 目的基于 Wnt 信号通路探究上调 mi-184 对糖尿病视网膜病变模型大鼠的干预效果。方法选取 40 只SD 健康大鼠，10 只作为正常组，剩余 30 只建立糖尿病视网膜病变模型，并随机分为模型组、下调 mi-184 组、上调 mi-184组。对 4 组大鼠分别进行干预，同时对 4 组大鼠视网膜组织进行苏木素-伊红(HE)染色处理。对总胆固醇(TC)、三酰甘油(TG)、空腹血糖水平进行检测对比。采用免疫比浊法、酶联免疫吸附试验分别对谷胱甘肽(GSH)、一氧化氮(NO)、血管内皮生长因子(VEGF)、肿瘤坏死因子(TNF)-水平进行检测。结果与正常组相比，模型组 TC、TG、空腹血糖、VEGF、TNF-、NO水平显著升高(P0.05);与模型组相比，下调 mi-184 组 TC、TG、空腹血糖、VEGF、TNF-、NO 水平显著升高(P0.05);与下调 mi-184 组相比，上调 mi-184 组 TC、TG、空腹血糖、VEGF、TNF-、NO 水平显著降低(P0.05);与正常组相比，模型组 GSH显著下降(P0.05);与模型组相比，下调 mi-184 组 GSH 显著下降(P0.05);与下调 mi-184 组相比，上调 mi-184 组 GSH显著上升(P0.05);与正常组相比，模型组糖原合成激酶(GSK)-3 表达显著升高、-连环蛋白(catenin)、原癌基因(C-Myc)表达显著降低(P0.05);与模型组相比，下调 mi-184 组 GSK-3 表达显著升高、-catenin、C-Myc 表达显著降低(P0.05);与下调 mi-184 组相比，上调 mi-184 组 GSK-3 表达显著降低，-catenin、C-Myc 表达显著升高(P0.05)。结论上调 mi-184 干预糖尿病视网膜病变模型大鼠，对血脂、血糖水平具有调控作用，可改善眼底视网膜环境，有效降低血管通透性，从而达到治疗糖尿病视网膜病变的效果。关键词 Wnt 信号通路;上调 mi-184;糖尿病视网膜病变;血糖;血脂中图分类号 587.2 文献标识码 A 文章编号 1005-9202(2023)03-0602-05;doi:10.3969/j.issn.1005-9202.2023.03.026基金项目:国家自然科学基金地区科学基金项目(82060886);贵州省自然科学基金资助项目(黔科合基础 2020 1Y366)通信作者:秦学维(1988-)，女，博士，主治医师，主要从事中西医结合治疗眼病研究。第一作者:郑丽(1987-)，女，医学硕士，主治医师，主要从事眼底病诊治研究。Intervention effect of up-regulated mi-184 on diabetic retinopathy modelrats based on Wnt signaling pathwayZHENG Li，QIN Xue-Wei，WANG Li-Min，et alDepartment of Ophthalmology，the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine，Guiyang206中国老年学杂志 2023 年 2 月第 43 卷550001，Guizhou，China【Abstract】ObjectiveTo explore the intervention effect of up-regulating mi-184 on diabetic retinopathy model rats based onWnt signaling pathway Methods40 SD healthy rats were selected，and 10 were selected as normal group，the remaining 30 were es-tablished diabetic retinopathy model，and randomly divided into model group，down-regulated mi-184 group and up-regulated mi-184 group The four groups of rats were intervened，and the retinal tissues of the four groups of rats were treated with hematoxylin-eosin(HE)staining at the same time The levels of total cholesterol(TC)，triacylglycerol(TG)and fasting blood glucose were detected andcompared The levels of glutathione(GSH)，nitric oxide(NO)，vascular endothelial growth factor(VEGF)and tumor necrosis factor(TNF)-were detected by immunoturbidimetry and enzyme-linked immunosorbent assay，respectively esultsCompared with thenormal group，the levels of TC，TG，fasting blood glucose，VEGF，TNF-and NO in model group were significantly increased(P0 05);compared with the model group，the levels of TC，TG，fasting blood glucose，VEGF，TNF-and NO in the down-regulatedmi-184 group were significantly increased(P0 05);compared with the down-regulated mi-184 group，the levels of TC，TG，fast-ing blood glucose，VEGF，TNF-and NO in the up-regulated mi-184 group were significantly decreased(P0 05);compared withthe normal group，the level of GSH in the model group was significantly decreased(P0 05);compared with the model group，the lev-el of GSH in the down-regulated mi-184 group was significantly increased(P0 05);compared with the down-regulated mi-184group，the level of GSH in the up-regulated mi-184 group was significantly increased(P0 05);compared with the normal group，the expression of glycogen synthase kinase(GSK)-3 was significantly increased and the expressions of-catenin(cateni