•专论与综述•(封面文章)doi:10.16801/j.issn.1008-7303.2022.0116基于RNA干扰的杀菌剂开发及其对化学杀菌剂的影响宋修仕#,高静#,周明国*(南京农业大学植物保护学院，南京210995)摘要：RNA干扰(RNAinterference,RNAi)是真核生物中高度保守的基因沉默现象，在医药与植物保护领域展现出广阔的应用潜力，相关产品已进入或即将进入医药与杀虫剂市场。近年来，科研工作者在基于RNAi技术的植物病原微生物的防控方面开展了大量研究，取得了进展，但仍无法实现基于RNAi防治植物病原真菌技术的商业化应用。本文概述了RNAi研究从1990年至今的发展历程，从细胞生物学、分子生物学角度提出了RNAi病害防控技术产品化瓶颈问题的新见解，同时讨论了基于RNAi的杀菌剂对传统化学杀菌剂的影响，可为RNAi杀菌剂的创制和应用提供参考。关键词:RNA干扰；杀菌剂；植物保护；瓶颈；挑战中图分类号：Q78；TQ458.7文献标志码：ADevelopmentoffungicidesbasedonRNAimechanismanditsinfluenceonchemicalfungicidesSONGXiushi#,GAOJing#,ZHOUMingguo*(CollegeofPlantProtectin,NanjingAgriculturalUniversity,Nanjing210995,China)Abstract:RNAinterference(RNAi)isahighlyconservedgenesilencingphenomenonineukaryotes,whichhasshownbroadpotentialapplicationinmedicineandplantprotection.Recently,RNAi-relatedproductshavebeenorabouttobeeninthepharmaceuticalandpesticidemarkets.ForRNAi-basedfungicides,althoughresearchershavemadealotofeffortsinthedevelopmentofitsproducts,thecommercialapplicationofRNAiagainstplantpathogenshasnotarrivedonthemarketyet.Here,thedevelopmentofRNAiresearchsince1990issummarized,andnewideasthatmayhelpimprovethedevelopmentofRNAi-basedfungicidesareintroduced.Atthesametime,theinfluenceofRNAi-basedfungicidesontraditionalchemicalfungicidesisdiscussed,whichistheinnovationandapplicationofRNAifungicides.Keywords:RNAi;fungicides;plantprotection;obstacles;challenges收稿日期：2022-08-22；录用日期：2022-10-04；网络首发日期：2022-10-09.Received:August22,2022;Accepted:October4,2022;Publishedonline:October9,2022.URL:https://doi.org/10.16801/j.issn.1008-7303.2022.0116http://www.nyxxb.cn/cn/article/doi/10.16801/j.issn.1008-7303.2022.0116基金项目：国家自然科学基金重点项目(31730072)；...
