中华中医药杂志(原中国医药学报)2023年2月第38卷第2期CJTCMP,February2023,Vol.38,No.2•620•基于产丁酸菌组合研究参苓白术散多糖体外降解的微生态机制林萍1,2，邱超1，舒青龙1，封勇1（1江西中医药大学中医学院，南昌330004；2解放军南部战区空军医院，广州510030）摘要：目的：研究“拟杆菌/厚壁菌”与产丁酸菌组合对中药多糖体外降解机制。方法：以参苓白术散多糖为唯一碳源构建多糖培养基（PM），脑心浸液培养基（BHI）为对照，通过连续取样测定产丁酸菌在PM、及不同菌（包括脆弱拟杆菌、多形拟杆菌、厚壁菌YT2等）的PM降解液中的生长曲线，评估多糖体外降解机制。结果：产丁酸菌在多形拟杆菌降解的PM降解液中生长最好，在PM中的生长最差；PM/BHI分别经多糖降解菌降解后，PM中的产丁酸菌菌群数量更高；丁酸浓度变化中，经厚壁菌YT2的PM降解液最高，PM中产丁酸浓度最低；经多糖降解菌降解的PM降解液>经多糖降解菌降解的BHI降解液。结论：“拟杆菌/厚壁菌”产丁酸菌组合有利于对中药多糖的降解利用和丁酸生产。关键词：产丁酸菌；参苓白术散；多糖；碳源；多糖降解菌基金资助：国家自然科学基金项目（No.82160788）StudyonthemicroecologicalmechanismofpolysaccharidedegradationofShenlingBaizhuPowderinvitrobasedonbutyricacidproducingbacteriacombinationLINPing1,2,QIUChao1,SHUQing-long1,FENGYong1(1CollegeofTraditionalChineseMedicine,JiangxiUniversityofChineseMedicine,Nanchang330004,China;2AirForceHospitalofSouthernTheaterCommandofPLA,Guangzhou510030,China)Abstract:Objective:TostudythedegradationmechanismoftraditionalChinesemedicinepolysaccharidesinvitrobythecombinationof‘Bacteroides/firmicum’andbutyricacidproducingbacteria.Polysaccharidemedium(PM)wasconstructedwiththepolysaccharideofShenlingBaizhuPowderastheonlycarbonsource,andbrainheartinfusionbroth(BHI)wasusedasthecontrol.Throughcontinuoussampling,thegrowthcurveofbutyricacidproducingbacteriainPMandPMdegradationsolutionofdifferentbacteria(includingBacteroidesfragilis,BacteroidesthetaiotaomicronandClostridiumsp.YT2,etc.)wasdeterminedweremeasuredtoevaluatethedegradationmechanismofpolysaccharidesinvitro.Results:ButyricacidproducingbacteriagrewbestinPMdegradationsolutiondegradedbyBacteroidesthetaiotaom...
