艾灸结合肺纤通络方对博莱霉...ad_3信号通路的调控作用_杨善军.pdf

第 39 卷第 3 期2023 年 3 月长春中医药大学学报Journal of Changchun University of Chinese MedicineVol.39 No.3Mar.2023278DOI:10.13463/ki.cczyy.2023.03.011艾灸结合肺纤通络方对博莱霉素所致肺纤维化大鼠的治疗效果及对 NF-B/TGF-1/smad 3 信号通路的调控作用 杨善军1，封 笑1，邢露茗1，王 玥1，曲 阳2*，王 浩1，李希尧3（1.黑龙江中医药大学第二附属医院肺病科，哈尔滨 150001；2.黑龙江中医药大学第二附属医院内科一诊室，哈尔滨 150001；3.北京中医药大学，北京 100029）摘要：目的目的 探究艾灸结合肺纤通络方对博莱霉素（BLM）所致肺纤维化（PF）大鼠的治疗效果及对 NF-B/TGF-1/smad 3 通路的调控作用。方法方法 从 50 只大鼠中随机选取 10 只为对照组，其余 40 只采用博莱霉素诱导建立 PF 模型，4只大鼠死亡，其余随机分为 PF 组、艾灸组、联合组及阳性组，各 9 只。艾灸组采用艾灸治疗，联合组在艾灸的穴位处滴加 5 滴肺纤通络方并艾灸至干燥，阳性组给予泼尼松 3 mLkg-1，PF 组及对照组给予等剂量的生理盐水。连续给药30 d 后，测量各组血清炎症因子及氧化应激指标水平，观察肺组织病理学变化，检测 NF-B/TGF-1/smad 3 通路蛋白表达情况。结果结果 与对照组比较，PF 组血清 IL-1、IL-6、TNF-水平及 HYP、GSH 水平升高，MDA 水平降低，NF-B、TGF-1、smad 3 mRNA 水平升高，NF-B、TGF-1、smad 3 蛋白表达上调（P 0.05）；与 PF 组比较，艾灸组、联合组及阳性组血清 IL-1、IL-6、TNF-水平及 HYP、GSH 水平降低，MDA 水平升高，NF-B、TGF-1、smad 3 mRNA 水平降低，NF-B、TGF-1、smad 3 蛋白表达下调（P 0.05）；与艾灸组比较，联合组及阳性组血清 IL-1、IL-6、TNF-及 HYP、GSH 水平降低，MDA 水平升高，NF-B、TGF-1、smad 3 mRNA 水平降低，NF-B、TGF-1、smad 3 蛋白表达下调（P 0.05）；与联合组比较，阳性组血清 IL-1、IL-6、TNF-水平及 HYP、GSH 水平降低，MDA 水平升高，NF-B、TGF-1、smad 3 mRNA 水平降低，NF-B、TGF-1、smad 3 蛋白表达下调（P 0.05）。结论结论 艾灸结合肺纤通络方能够改善 BLM 所致 PF 大鼠炎症指标及氧化应激指标，对肺组织起到保护作用，其作用可能与 NF-B/TGF-1/smad 3 通路有关。关键词：艾灸；肺纤通络方；博莱霉素；肺纤维化；NF-B/TGF-1/smad 3 通路；大鼠中图分类号：R563.9 文献标志码：A 文章编号：2095-6258(2023)03-0278-05Therapeutic effect of moxibustion combined with Feixian Tongluo decoction on Therapeutic effect of moxibustion combined with Feixian Tongluo decoction on bleomycin-induced pulmonary fibrosis in rats and its regulatory effect on the NF-B/bleomycin-induced pulmonary fibrosis in rats and its regulatory effect on the NF-B/TGF-TGF-1 1/smad 3 pathway/smad 3 pathwayYANG Shanjun1,FENG Xiao1,XING Luming1,WANG Yue1,QU Yang2*,WANG Hao1,LI Xiyao3(1.Department of Pulmonary Diseases,Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150001,China;2.Department of Internal Medicine,Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150001,China;3.Beijing University of Chinese Medicine,Beijing 100029,China)Abstract:Objective Abstract:Objective To investigate the therapeutic effect of moxibustion combined with Feixian Tongluo decoction on bleomycin 基金项目：黑龙江省自然科学基金项目（LH2020H100）；黑龙江省中医药科研项目（ZHY2020-136）；哈尔滨市科技创 新人才研究专项资金（青年后备人才公益类）（2017RAQXJ189）；黑龙江中医药大学附属第二医院横向课题 （HX201901）作者简介：杨善军（1978），男，硕士，副主任医师，主要从事肺部疾病方面研究*通信作者：曲 阳，男，主治医师，电子信箱-第 39 卷第 3 期2023 年 3 月长春中医药大学学报Journal of Changchun University of Chinese MedicineVol.39 No.3Mar.2023279(BLM)induced pulmonary fibrosis(PF)in rats and its regulatory effect on the NF-B/TGF-1/smad 3 pathway.MethodsMethods Ten of 50 rats were randomly selected as the control group,and the other 40 rats were induced by bleomycin to establish a PF model,among which 4 rats died,and the rest were randomly divided into a PF group,a moxibustion group,a combination group and a positive group,with 9 rats in each group.The moxibustion group was treated with moxibustion.In the combination group,5 drops of Feixian Tongluo decoction were administered to the acupoints where moxibustion was carried out until they got dry.The positive group was treated with prednisone 3mLkg-1,and the PF group and the control group were given the equal dose of normal saline.After 30 days of continuous administration,the levels of serum inflammatory factors and oxidative stress indexes were measured,the pathological changes of lung tissues were observed,and the protein expression of the NF-B/TGF-1/smad 3 pathway was detected.ResultsResults Compared with the control group,the serum levels of IL-1,IL-6,TNF-,the levels of HYP and GSH were increased in the PF group,while the level of MDA was decreased,and the mRNA levels of NF-B,TGF-1 and smad 3 were increased,the protein expression levels of NF-B,TGF-1 and smad 3 were up-regulated(P0.05).Compared with the PF group,in the moxibustion group,the combination group and the positive group,the serum levels of IL-1,IL-6 and TNF-,the levels of HYP and GSH were decreased,the level of MDA was increased,and the mRNA levels of NF-B,TGF-1 and smad 3 were decreased,while the protein expression levels of NF-B,TGF-1 and smad 3 were down-regulated(P0.05).Compared with the moxibustion group,in the combination group and the positive group,the serum levels of IL-1,IL-6,TNF-,the levels of HYP and GSH were decreased,the level of MDA was increased,the mRNA levels of NF-B,TGF-1 and smad 3 were decreased,and the protein expression levels of NF-B,TGF-1 and smad 3 were down-regulated(P0.05).Compared with the combination group,the serum levels of IL-1,IL-6 and TNF-,the levels of HYP and GSH in the positive group were decreased,the level of MDA was increased,the mRNA levels of NF-B,TGF-1 and smad 3 were decreased,and the protein expression levels of NF-B,TGF-1 and smad 3 were down-regulated(P0.05).ConclusionConclusion Moxibustion combined with Feixian Tongluo decoction can improve the inflammatory indexes and oxidative stress indexes of BLM-induced PF rats,and play a role in protecting lung tissues,whic