作者简介:郭巍(１９８１—),男,焦作大学讲师.EＧmail:bitachun７４０７００＠１６３．com收稿日期:２０２２Ｇ０２Ｇ１１改回日期:２０２２Ｇ０９Ｇ２３DOI:１０．１３６５２/j．spjx．１００３．５７８８．２０２２．９０２４１[文章编号]１００３Ｇ５７８８(２０２３)０１Ｇ００４２Ｇ０５HILICＧMS/MS法检测动物源运动营养品中９种抗病毒药物残留Determinationoftheresiduesof９kindsofantiviraldrugsinanimalＧderivedsportsnutritionbyHILICＧMS/MS郭巍GUOWei(焦作大学,河南焦作４５４０００)(JiaozuoUniversity,Jiaozuo,Henan４５４０００,China)摘要:目的:加强动物源性运动营养品中抗病毒药物残留的监控.方法:建立一种亲水交互作用色谱—串联质谱测定动物源运动营养品中９种抗病毒药物组分的方法.样品前处理采用１％乙酸—乙腈提取,经PRiMEHLB小柱净化后检测.采用乙腈—１０mmol/L乙酸铵溶液(含０．１％甲酸)流动相体系,在梯度洗脱模式下,经SielcObeliscR柱分离,实现９种目标物组分的分离.结果:９种抗病毒药物组分在０．１~２０．０ng/mL质量浓度范围内线性良好,检出限为０．１~０．５μg/kg,定量限为０．３~１．５μg/kg,加标回收率达８２．３％~９５．７％,相对标准偏差为３．２％~５．９％(n＝５).结论:试验方法可以满足动物源运动营养品中９种抗病毒药物残留的检测需求.关键词:固相萃取;亲水色谱;动物源;运动营养品;抗病毒类药物Abstract:Objective:ThisstudyaimedtohelpwithstrengtheningthemonitoringofantiviraldrugresiduesinanimalＧderivedsportsnutritionproducts．Methods:Amethodfordeterminingtheresiduesof９kindsofantiviraldrugsinanimalＧderivedsportsnutritionwasestablished,byusinghydrophilicinteractionchromatographytandemmassspectrometry．Thesampleswereextractedby１％aceticacidＧacetonitrileandpurifiedbyPRiMEHLBcolumn．ThemobilephasesystemofacetonitrileＧ１０mmol/Lammoniumacetatesolution(containing０．１％formicacid)wasseparatedbySielcObeliscRcolumningradientelutionmodetoachievetheseparationofninetargetcomponents．Results:The９kindsofantiviraldrugsfractionshadgoodlinearityinthemassconcentrationrangeof０．１~２０．０ng/mL,andthelimitofdetectionwas０．１~０．５μg/kg,withthelimitofquantificationof０．３~１．５μg/kgandthespikedrecoveryof８２．３％~９５．７％,andt...
