·1582·中草药2023年3月第54卷第5期ChineseTraditionalandHerbalDrugs2023MarchVol.54No.5HPLC测定壮药羊开口中3,3′,4′-三甲氧基鞣花酸-4-O-β-D-吡喃葡萄糖苷的含量5刘立辉，黄丽玲，唐林波，常华，邹洵*桂林三金药业股份有限公司，广西桂林541004摘要：目的建立HPLC测定壮药羊开口中3,3′,4′-三甲氧基鞣花酸-4-O-β-D-吡喃葡萄糖苷的含量方法。方法采用WelchMaterialsUltimateAQC18反相色谱柱（250mm×4.6mm，5μm）；流动相为乙腈-0.2%磷酸水溶液，采用梯度洗脱，体积流量为1.0mL/min，检测波长为246nm，柱温为35℃。结果3,3′,4′-三甲氧基鞣花酸-4-O-β-D-吡喃葡萄糖苷进样量在0.02～0.60μg内与峰面积线性关系良好（r＝1.0000）；平均回收率为105.58%，RSD为2.27%。结论该方法简便、重复性好、准确度高，可用于壮药羊开口的质量控制。关键词：羊开口；野牡丹；3,3′,4′-三甲氧基鞣花酸-4-O-β-D-吡喃葡萄糖苷；HPLC；质量控制中图分类号：R286.2文献标志码：A文章编号：0253-2670(2023)05-1582-04DOI:10.7501/j.issn.0253-2670.2023.05.025Determinationof4-O-β-D-glucopyranosyl-3,3′,4′-tri-O-methylellagicacidinZhuangMedicineRadixMelastomatisNormalisbyHPLCLIULi-hui,HUANGLi-ling,TANGLin-bo,CHANGHua,ZOUXunGuilinSanjinPharmaceuticalCo.,Ltd.,Guilin541004,ChinaAbstract:ObjectiveToestablishaHPLCmethodforthedeterminationof4-O-β-D-glucopyranosyl-3,3′,4′-tri-O-methylellagicacidinZhuangMedicineRadixMelastomatisnormalis.MethodsAWelchMaterialsUltimateAQC18reversephasecolumn（250mm×4.6mm,5μm）wasused.Themobilephaseconsistedofacetonitrile-watercontaining0.2%phosphoricacidsolutionwithgradientelutionataflowrateof1.0mL/min.Thedetectionwavelengthwassetat246nmandthetemperatureofcolumnwas35℃.ResultsThelinearrelationshipbetween4-O-β-D-glucopyranosyl-3,3′,4′-tri-O-methylellagicacidinjectionamountandpeakareawasgoodintherangeof0.02—0.60μg(r＝1.0000).Theaveragerecoverywas105.58%withRSDof2.27%.ConclusionThemethodissimple,reproducibleandaccurate,andcanbeusedforthequalitycontrolofZhuangMedicineRadixMelastomatisnormalis.Keywords:RadixMelastomatisnormalis;MelastomanormaleD.Don;4-O-β-D-glucopyranosyl-3,3′,4′-tri-O-methylellagicacid;HPLC;qualitycon...
