肿瘤防治研究2023年第50卷第1期CancerResPrevTreat,2023,Vol.50,No.1·12·doi:10.3971/j.issn.1000-8578.2023.22.0535阿克替苷通过调控ERK1/2信号通路抑制肝癌细胞上皮间质转化袁倩倩，何昱静，温雪，张久聪，郑英，卢利霞，李斌，于晓辉ActeosideInhibitsEpithelialMesenchymalTransformationofHepatomaCellsThroughRegulationofERK1/2SignalingPathwayYUANQianqian,HEYujing,WENXue,ZHANGJiucong,ZHENGYing,LULixia,LIBin,YUXiaohuiDepartmentofGastroenterology,The940thHospitalofJointServiceLogisticsSupportForceofChinesePeople’sLiberationArmy,Lanzhou730050,ChinaCorrespondingAuthor:YUXiaohui,E-mail:yuxiaohui528@126.comAbstract:ObjectiveToinvestigatetheeffectandmechanismofacteoside(ACT)ininhibitingepithelial-mesenchymaltransition(EMT)inhumanhepatomaHCCLM3cellsbyregulatingtheERK1/2pathway.MethodsCCK-8assaywasusedtodetecttheeffectofhepatocellularcarcinomacellproliferation.TheinvasionandmigrationofHCCcellsweredetectedbyscratchandTranswelltests.ThemRNAandproteinexpressionlevelsoftheERK1/2signalingpathwayandEMT-relatedgenes(E-cadherinandN-cadherin)weredetectedbyreal-timePCRandWesternblotanalyses.ResultsACTreducedtheactivityofHCCLM3cellsandinhibitedtheproliferationofHCCcells,andtheeffectshadcertaincorrelationwithdrugconcentrationandtime.ACTinhibitedthemigrationandinvasionprocessofHCCLM3cellsinaconcentration-dependentmanner.ACTdownregulatedthemRNAandproteinexpressionofgenesrelatedtotheERK1/2signalingpathway.ItincreasedthemRNAandproteinexpressionlevelsoftheEMT-relatedgeneE-cadherinbutdecreasedthoseofN-cadherin.ConclusionACTcouldinhibitEMTandtheinvasionandmigrationofHCCLM3cellsinhumanhepatoma,andtheunderlyingmechanismiscloselyrelatedtothedownregulationoftheERK1/2signalingpathway.Keywords:Acteoside;HCCLM3cells;ERK1/2signalingpathway;Epithelialmesenchymaltransformation;Invasion;MigrationFunding:GansuProvincialScienceandTechnologyDepartmentSocialDevelopmentDepartmentClinicalMedicalResearchCenterProject(No.21JR7RA017;No.20JR10RA017)Competinginterests:Theauthorsdeclarethattheyhavenocompetinginterests.摘要：目...
