195现代检验医学杂志第38卷第1期2023年1月JModLabMed,Vol.38,No.1,Jan.20231例骨髓增生异常综合征多次受血患者血清存在抗-M,抗-cE和抗-Jkb抗体的鉴定及配血输血策略张良子,左琴琴,吴大洲,褚晓月,毛娟,张薇薇,王红,徐华(陕西省血液中心,西安710061)摘要:目的通过对临床送检的1例骨髓增生异常综合征(myelodysplasticsyndrome,MDS)多次受血患者的抗筛阳性标本进行鉴定,探讨此类复杂不规则抗体的鉴定思路、方法及输血方案。方法鉴定标本ABO,Rh,MNS和Kidd等血型,应用2种谱细胞及筛选出的无偿献血者细胞配合吸收放散实验进行抗体筛查、复杂不规则抗体特异性鉴定,采用盐水法、凝聚胺法和间接抗球蛋白方法进行交叉配血。结果患者血型血清学结果为O,CCDee,M-N+和Jk(a+b-),其血清中检出IgM-M,IgG-cE和IgG-Jkb抗体,筛选M,c,E及Jkb抗原均为阴性的供血者与患者进行交叉配血,配血相合后进行输注。结论针对多次受血患者产生的复杂不规则抗体,应灵活运用灵敏度高的血清学方法,联合多种谱细胞,准确鉴定出抗体特异性,为临床提供相合的血液,有效保障输血的安全性。关键词:骨髓增生异常综合征;抗-M;抗-Jkb中图分类号:R551.3;R457.12文献标识码:A文章编号:1671-7414(2023)01-195-04doi:10.3969/j.issn.1671-7414.2023.01.037IdentificationandTransfusionStrategyofAnti-M,Anti-cEandAnti-JkbAntibodiesintheSerumofPatientswithMyelodysplasticSyndromesReceivingMultipleBloodTransfusionsZHANGLiang-zi,ZUOQin-qin,WUDa-zhou,CHUXiao-yue,MAOJuan,ZHANGWei-wei,WANGHong,XUHua(BloodCenterofShaanxiProvince,Xi’an710061,China)Abstract:ObjectiveToidentifytheanti-screeningpositivespecimensofapatientwithmyelodysplasticsyndrome(MDS)whoreceivedmultiplebloodsamples,andexploretheidentificationideas,methodsandtransfusionschemesofsuchcomplexirregularantibodies.MethodsIdentifiedtheABO,Rh,MNSandKiddbloodgroups,screenedandidentifiedthemixedirregularantibodiesthroughabsorptionandelutiontestwithvariouscellpanels.Crossmatchingwasperformedbysalinemethod,polyaminemethodandindirectantiglobulinmethod.ResultsThebloodtypeswereO,CCDee,M-N+,Jk(a+b-).IgM-M,IgG-cEandIgG-Jkbantibodiesweredetectedintheserumofpatientsincontrolofthecellspectrumresponsepattern.Theblooddonorswhower...