白花泡桐果皮和花芽的化学成分研究_张文超.pdf

参考文献1 李敏娜.新型免疫抑制剂雷帕霉素的研究进展J.河北化工,2011,34(8):21-22,24.2 杨婧,张小东,杨辉,等.肾移植术后应用雷帕霉素转换方案的临床观察J.中国医院药学杂志,2018,38(6):650-653.3 刘现忠,王轩.雷帕霉素抗排斥作用机制及其在肝移植中的应用J.免疫学杂志,2014,30(1):79-83.4 Dai Y,Wang L,Fu L,et al.The efficacy and safety of domestic and imported rapamycin drug-eluting stents and paclitaxel drug-coated balloons in the treatment of coronary bifurcation lesionsJ.Am J Transl Res,2021,13(8):9421-9428.5 Juwana Y B,Rasoul S,Ottervanger J P,et al.Efficacy and safety of rapamycin as compared to paclitaxel-eluting stents:a meta-analysisJ.J Invasive Cardiol,2010,22(7):312-316.6 Towner R A,Gulej R,Zalles M,et al.Rapamycin restores brain vasculature,metabolism,and blood-brain barrier in an inflammaging modelJ.Geroscience,2021,43(2):563-578.7 Phillips B N,Wroblewski K J.A retrospective review of oral low-dose sirolimus(rapamycin)for the treatment of active uveitisJ.J Ophthalmic Inflamm Infect,2010,1(1):29-34.8 Ehninger D,Neff F,Xie K.Longevity,aging and rapamycinJ.Cell Mol Life Sci,2014,71(22):4325-4346.9 Selvarani R,Mohammed S,Richardson A.Effect of rapamycin on aging and age-related diseases-past and futureJ.Geroscience,2021,43(3):1135-1158.10 Harrison D E,Strong R,Sharp Z D,et al.Rapamycin fed late in life extends lifespan in genetically heterogeneous miceJ.Nature,2009,460(7253):392-395.11 Zhao Y,Liu Y,Zhou L,et al.Trends of rapamycin in survival benefits of liver transplantation for hepatocellular carcinomaJ.World J Gastrointest Surg,2021,13(9):953-966.12 Sun C Y,Li Y Z,Cao D,et al.Rapamycin and trametinib:a rational combination for treatment of NSCLCJ.Int J Biol Sci,2021,17(12):3211-3223.13 Faes S,Demartines N,Dormond O.Mechanistic target of rapamycin inhibitors in renal cell carcinoma:potential,limitations,and perspectivesJ.Front Cell Dev Biol,2021,(9):636037.14 Querfurth H,Lee H K.Mammalian/mechanistic target of rapamycin(mTOR)complexes in neurodegenerationJ.Mol Neurodegener,2021,16(1):1-25.15 山东省药学科学院.一种高产雷帕霉素的吸水链霉菌：CN,201811305747.2 P.2021-08-0616 El-Gendy N,Madian H R,Amr S S.Design and optimization of a process for sugarcane molasses fermentation by saccharomyces cerevisiae using response surface methodologyJ.Int J Microbiol,2013,2013(2):815631.17 Chandra M,Barata A,Ferreira-Dias S,et al.A Response Surface Methodology study on the role of factors affecting growth and volatile phenol production by Brettanomyces bruxellensis ISA 2211 in wineJ.Food Microbiol,2014,42:40-46.18 Kim Y H,Park B S,Bhatia S K,et al.Production of rapamycin in Streptomyces hygroscopicus from glycerol-based media optimized by systemic methodologyJ.J Microbiol Biotechnol,2014,24(10):1319-1326.收稿日期：2021-12-30基金项目：山东省中医药科技项目（编号：2021M154）；山东省农业科技资金（园区产业提升工程）项目（编号：2019YQ03）作者简介：张文超，硕士研究生，研究方向为天然药物化学，E-mail:*通讯作者：唐文照，教授，硕士生导师，研究方向为天然药物化学，E-mail:白花泡桐果皮和花芽的化学成分研究张文超1，吴素英2，张梦蛟1，贾献慧1，唐文照1*（1.山东第一医科大学 药学与制药科学学院，山东 济南 250117；2.山东中医药大学，山东 济南 250355）摘 要:目的 研究白花泡桐果皮和花芽的化学成分。方法 采用硅胶、SephadexLH-20、ODS等柱色谱层析方法对白花泡桐果皮和花蕾的95%乙醇提取物进行分离纯化，根据化合物的波谱分析（MS，CD，1HNMR与13CNMR）进行结构鉴定。结果 从白花泡桐果皮中分离纯化得到6个化合物，分别鉴定为：paulownione A（I），(2R,3R)-4,5,5,7-tetrahydroxy-3-methoxy-6-6-hydroxy-3,7-dimethyl-2(E),7-octadienylflavanone（II），tanariflavanone D（III），isopaucatalinone B（IV），prokinawan（V），洋芹素（VI）。从其花芽中分离纯化得到12个化合物，分别鉴定为：diplacone（VII），3-O-methyl-5-methoxy-diplacol（VIII），diplacol（IX），异槲皮苷（X），金圣草黄素（XI），dihydrotricin（XII），(-)-芝麻素（XIII），(+)-松脂素（XIV），(erythro)-longifloroside B（XV），(threo)-longifloroside B（XVI），毛蕊花糖苷（XVII），异毛蕊花糖苷（XVIII）。结论 其中化合物XV和XVI为首次从泡桐属中分离得到，化合物I、II、III、IV、V、VIII、IX、食品与药品 Food and Drug 2023年第25卷第1期 5XI、XII和XIV为首次从白花泡桐植物中分离得到。此外，白花泡桐的花芽中苯乙醇苷类化合物的含量较高，可作为该类化合物（毛蕊花糖苷）的植物资源进行开发利用。关键词:泡桐属；白花泡桐;化学成分；苯乙醇苷中图分类号：R284 文献标识码：A 文章编号：1672-979X（2023）01-0005-08DOI：10.3969/j.issn.1672-979X.2023.01.002Study on Chemical Constituents of Paulownia Fortunei Peel and BudZHANG Wen-chao1,WU Su-ying2,ZHANG Meng-jiao1,JIA Xian-hui1,TANG Wen-zhao1(1.School of Pharmacy and Pharmaceutical Science,Shandong First Medical University,Jinan 250117,China;2.Shandong University of Traditional Chinese Medicine,Jinan 250355,China)Abstract:Objective To investigate the chemical constituents from the peel and bud of Paulownia fortunei.Methods Compounds were isolated and purified from 95%ethanol extracts of P.fortunei peel and bud by silica gel,Sephadex LH-20 and ODS column chromatography,and their structures were identified on the basis of spectral analysis(MS,CD,1HNMR and 13CNMR).Results Six compounds were isolated from the peel of Paulownia fortunei and identified as:paulownione A(I),(2R,3R)-4,5,5,7-tetrahydroxy-3-methoxy-6-6-hydroxy-3,7-dimethyl-2(E),7-octadienylflavanone(II),tanariflavanone D(III),isopaucatalinone B(IV),prokinawan(V),apigenin(VI).Twelve compounds were obtained from its bud and identified as:diplacone(VII),3-O-methyl-5-methoxy-diplacol(VIII),diplacol(IX),isoquercitrin(X),chrysoeriol(XI),dihydrotricin(XII),(-)-sesamin(XIII),(+)-pinoresinol(XIV),(erythro)-longifloroside B(XV),(threo)-longifloroside B(XVI),acteoside(XVII),isoacteoside(XVIII).Conclusion Compounds XV and XVI were isolate