肿瘤防治研究2023年第50卷第1期CancerResPrevTreat,2023,Vol.50,No.1·27·doi:10.3971/j.issn.1000-8578.2023.22.0705RBM8A基因对子宫内膜癌HEC-1A细胞增殖、迁移和凋亡的作用及其机制谭冬梅1，张静静2，师一民1，韩赛2，耿炜1，孙建一1，王雅玉1，张秀荣1EffectandMechanismofRBM8AonProliferation,MigrationandApoptosisofHumanEndometrialCancerHEC-1ACellsTANDongmei1,ZHANGJingjing2,SHIYimin1,HANSai2,GENGWei1,SUNJianyi1,WANGYayu1,ZHANGXiurong11.TCMGynecology,ShandongProvincialMaternalandChildHealthCareHospital,Ji’nan250000,China;2.DepartmentofObstetricsandGynecology,QiluHospitalofShandongUniversity,Ji’nan250000,ChinaCorrespondingAuthor:ZHANGXiurong,E-mail:18615585827@163.comAbstract:ObjectiveToinvestigatetheeffectofsilencedRBM8Ageneonthebiologicalbehavior(proliferation,migration,andapoptosis)ofhumanendometrialcancerHEC-1Acellsanditspossiblemechanism.MethodsThehairpinshRNAtargetedbytheRBM8Agenewasdesigned,andthebestshRNAsilencingfragmentwasscreened.TherecombinantlentiviralinterferencevectorcarryingthetargetgenewasconstructedandusedtoinfectHEC-1Acells.CellswithstableknockdownofRBM8Agenewerescreenedbypuromycinastheexperimentalgroup(shRBM8A),whiletheshRNAofnonsensesequencewasdesignedasthecontrolgroup(shControl).CCK-8methodwasusedtodetectcellproliferation,andflowcytometrywasusedtodetectcellapoptosis.Transwellassaywasusedtodetectcellmigrationandinvasion.Westernblotwasusedtoanalyzetheexpressionofapoptosis-relatedproteinsandEMTsignaltransductionpathwayrelatedproteins.ResultsIncomparisonwiththeshControlgroup,afterRBM8Aknockdown,HEC-1Acellproliferationwasreduced,apoptosiswasincreased,migrationandinvasionabilityweresignificantlyinhibited(P<0.05),theexpressionofapoptosis-relatedproteinscleavedcaspase9andcaspase3increased,EMT-relatedproteinE-cadherinexpressionincreased,andVimentinexpressiondecreased.ConclusionRBM8Agenesilencingcaninhibittheproliferation,migration,andinvasionandpromotetheapoptosisofendometrialcancercells.TheinhibitionofEMTsignaltransductionpathwaymaybeitsmechanism.Keywords:Humanend...
