·基础研究·LncRNALINC00663对胶质瘤细胞增殖及凋亡的影响时景仁,郭晶晶,王爽,王雅杰(首都医科大学附属北京地坛医院检验科,北京100015)DOI:10.11748/bjmy.issn.1006-1703.2022.11.024收稿日期:2022-01-02;修回日期:2022-01-25基金项目:国家自然科学基金(编号:81572474)通讯作者:王雅杰,首都医科大学附属北京地坛医院检验科,教授。摘要:目的探讨长链非编码RNA(LncRNA)-LINC00663对胶质瘤细胞增殖和凋亡的影响,并探讨其可能机制。方法实时荧光定量PCR检测人脐静脉内皮细胞(HUVEC)和胶质瘤细胞系A172中LINC00663的表达水平。用siRNA沉默A172细胞中LINC00663的表达,分为siRNA-NC组和siRNA-LINC组,采用CCK-8法和EDU法检测细胞增殖,流式细胞术AnnexinV-FITC/7-AAD双染法检测细胞凋亡率,Westernblot检测凋亡相关蛋白Bax和Bcl-2的表达水平,Westernblot检测细胞内Wnt3a表达水平。结果A172细胞中LINC00663的表达水平明显高于HUVEC(P<0.01)。与siRNA-NC组相比,siRNA-LINC组细胞增殖能力降低,凋亡率增加,Wnt3a表达水平降低,差异具有统计学意义(P<0.05)。结论LINC00663可能通过调控Wnt3a表达影响A172细胞的增殖和凋亡。关键词:胶质瘤;LINC00663;增殖;凋亡;Wnt3a中图分类号:R739.41文献标识码:AEffectsofLncRNALINC00663onProliferationandApoptosisofHumanGliomaCellsSHIJingren,GUOJingjing,WANGShuang,WANGYajie(BeijingDitanHospital,CapitalMedicalUniversity,Beijing100015,China)Abstract:ObjectiveToinvestigatetheeffectsoflongnon-codingRNA(LncRNA)-LINC00663onproliferationandapoptosisinhumangliomacelllineA172andtheunderlyingmolecularmechanism.MethodsTheexpressionofLINC00663inhumanumbilicalveinendothelialcelllineHUVECandhumangliomacelllineA172wasdeterminedbyreal-timefluorescencequantitativePCR.TheexpressionofLINC00663inA172cellswassilencedbysiRNA,thencellsweredividedinthesiRNA-NCgroupandsiRNA-LINCgroup.CellcountingKit-8andEdUassaywereadoptedfortheanalysisofcellproliferation.Cellapoptosiswereevaluatedbyflowcytometry.TheexpressionlevelsofBAX,BCL-2,andWnt3aweredeterminedbywesternblotting.ResultsTheexpressionlevelofLINC00663inA172cellswassignificantlyhigherthanthatinHUVEC(P<0.01).ComparedwiththesiRNA-NCgroup,t...
