毕节地区刺梨多糖提取工艺优...糖含量与抗氧化活性差异研究_刘欣.pdf

第 14 卷 第 2 期 食 品 安 全 质 量 检 测 学 报 Vol.14 No.2 2023 年 1 月 Journal of Food Safety and Quality Jan.,2023 基金项目:广州市科技计划项目(202002020006)、广东省高校特色创新项目(2018KTSCX093)、广东省农村科技特派员项目(粤科函社农字20182136 号)、广州市农村科技特派员项目(GZKTP202019)、广东省普通高校重点实验室项目(2020KSYS005)Fund:Supported by the Science and Technology Project of Guangzhou(202002020006),the Characteristic Innovation Projects in Colleges and Universities in Guangdong Province(2018KTSCX093),the Rural Science and Technology Correspondent Workstation of Guangdong Province(YueKeHanSheNongZi2018No.2136),the Rural Science and Technology Correspondent Workstation of Guangzhou City(GZKTP202019),and the Guangdong University Key Laboratory for Substanable Control of Fruit and Vegetable Diseases and Pests(2020KSYS005)*通信作者:程杏安,博士,副教授,主要研究方向为天然产物化学。E-mail:anzai_*Corresponding author:CHENG Xing-An,Ph.D,Associate Professor,Zhongkai University of Agriculture and Engineering,No.24,Dongsha street,Haizhu District,Guangzhou 510225,China.E-mail:anzai_ 毕节地区刺梨多糖提取工艺优化及产地间 多糖含量与抗氧化活性差异研究 刘 欣1,李俊杰1,邹启文1,蒋旭红1,陈雪曼1,陈锦松1,程杏安1,2*,成忠均3,李恒谦3(1.仲恺农业工程学院化学化工学院,广州 510225;2.农业农村部华南果蔬绿色防控重点实验室,广州 510225;3.毕节市中药研究所,毕节 551799)摘摘 要要:目的目的 优化超声辅助水提醇沉法提取刺梨多糖的工艺条件,探究不同产地与品种间的刺梨多糖含量及抗氧化活性差异。方法方法 以提取率为指标,在单因素试验基础上,通过正交试验对超声辅助水提醇沉法工艺条件进行优化;并以该工艺对毕节地区不同产地与品种的刺梨样品进行多糖含量的测定与分析,对 1,1-二苯基-2-苦基肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基和 2,2-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt,ABTS阳离子自由基清除能力进行测定,比较各样品多糖的抗氧化活性。结果结果 超声辅助水提醇沉法提取刺梨多糖的最优工艺为:提取温度 75,提取时间 2.5 h,料液比 1:60(g/mL);其最佳提取率为 11.88%。对 37 个产地的 3 个品种间多糖含量分析可知,野生品种的多糖含量整体更高,为 40.87%,纳雍县的多糖含量整体最高,为 41.40%,刺梨多糖含量与海拔高度间未见规律性变化。各刺梨多糖对DPPH的半抑制浓度(half maximal inhibitory concentration,IC50)明显小于对ABTS+的IC50,抗氧化活性未见与海拔高度存在直接相关。结论结论 优化后的超声辅助水提醇沉提取工艺高效可行,各产地间多种刺梨品种多糖含量的高低呈现出一定的品种倾向性与区域偏向性,其多糖抗氧化活性也存在较大的差异,这可为刺梨在地区间的精准栽培及选种育种提供参考,为刺梨资源在功能食品开发等方面提供技术和理论支持。关键词关键词:刺梨;多糖;提取工艺;抗氧化活性 Optimization of high-efficiency extraction of polysaccharide from Rosa roxburghii and study on the difference of polysaccharide content and antioxidant activity among different regions in Bijie LIU Xin1,LI Jun-Jie1,ZOU Qi-Wen1,JIANG Xu-Hong1,CHEN Xue-Man1,CHEN Jin-Song1,CHENG Xing-An1,2*,CHENG Zhong-Jun3,LI Heng-Qian3(1.College of Chemistry and Chemical Engineering,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;2.Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs,P.R.China,Guangzhou 510225,China;3.Bijie Institute of Traditional Chinese Medicine,Bijie 551799,China)DOI:10.19812/ki.jfsq11-5956/ts.2023.02.044298 食 品 安 全 质 量 检 测 学 报 第 14 卷 ABSTRACT:Objective To optimize the extraction conditions of Rosa roxburghii polysaccharides by ultrasonic assisted water extraction and alcohol precipitation,and explore the differences in content and antioxidant activity of polysaccharide from Rosa roxburghii from different origins and varieties.Methods On the basis of single factor experiment,the technology of ultrasonic assisted water extraction and alcohol precipitation was optimized by orthogonal design with the extraction rate as the index.The polysaccharide content of Rosa roxburghii samples from different areas and varieties in Bijie was determined and analyzed by this process.Meanwhile,the antioxidant activity of polysaccharides from each sample was compared through the difference in the scavenging activity of 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)cationic free radical.Results The optimum extraction conditions of Rosa roxburghii polysaccharide by ultrasonic assisted water extraction and alcohol precipitation were as follows:Extraction temperature 75,extraction time 2.5 h,material liquid ratio 1:60(g/mL).The optimum extraction rate was 11.88%.According to the analysis of the polysaccharide content among the 3 varieties from 37 producing areas,the polysaccharide content of wild varieties was higher as a whole,which was 40.87%,the polysaccharide content of Nayong County was the highest,41.40%.There was no regular change between the polysaccharide content of Rosa roxburghii and the altitude.The half maximal inhibitory concentration(IC50)of each Rosa roxburghii polysaccharide on DPPH was significantly lower than that of ABTS+.The antioxidant activity was not directly related to the altitude.Conclusion The ultrasonic assisted water extraction and alcohol precipitation extraction process optimized is efficient and feasible.It is known that the content of polysaccharides of various Rosa roxburghii varieties in different regions shows a certain variety bias and regional bias.Their antioxidant activities also has a large difference.This can provide a reference for the precise cultivation and selection breeding of Rosa roxburghii in different regions.Besides,it can provide a reference for the utilization of Rosa roxburghii resources in food storage,and provide a technological and theoretical suppor