农业环境科学学报JournalofAgro⁃EnvironmentScience2022，41（12）:2824-28302022年12月李超楠，李洪涛，李运朝，等.北苍术根腐病病原菌分离鉴定及其生防菌筛选[J].农业环境科学学报,2022,41（12）：2824-2830.LICN,LIHT,LIYC,etal.IsolationandidentificationofrootrotpathogenofAtractylodeschinensisandscreeningofbiocontrolbacteria[J].JournalofAgro-EnvironmentScience,2022,41（12）：2824-2830.开放科学OSID北苍术根腐病病原菌分离鉴定及其生防菌筛选李超楠1，2，李洪涛2*，李运朝2，李俊花2，及华2，章丽2，王琳2（1.河北农业大学植物保护学院，河北保定071000；2.河北省农林科学院生物技术与食品科学研究所，石家庄050051）IsolationandidentificationofrootrotpathogenofAtractylodeschinensisandscreeningofbiocontrolbacteriaLIChaonan1,2,LIHongtao2*,LIYunchao2,LIJunhua2,JIHua2,ZHANGLi2,WANGLin2（1.CollegeofPlantProtection,HebeiAgriculturalUniversity,Baoding071000,China;2.InstituteofBiotechnologyandFoodScience,HebeiAcademyofAgricultureandForestrySciences,Shijiazhuang050051,China）Abstract：ToidentifythepathogenofrootrotofAtractylodeschinensis,pathogenicmicroorganismswereisolatedfromthetuberofAtractylodeschinensisinChengde,andthepathogenicityofthepathogenicmicroorganismswasverifiedviaback-grafting.TheisolatedpathogenicbacteriumwithpathogenicitywasidentifiedasFusariumoxysporum.Atotalof94rhizospheremicroorganismswereisolatedfromtherhizospheresoilofhealthymedicinalplants,andtwobiocontrolstrains,strainSFJ-27andstrainSFJ-41,wereobtainedthroughplateantagonismscreening.Plateantagonismtestresultsshowedthattheinhibitionratesofthetwobiocontrolstrainswereabove50%.ItwasidentifiedasBacillussubtilisandBacillusvelezensisaccordingtomolecularbiologyandmorphology.PotexperimentsshowedthatstrainSFJ-27andstrainSFJ-41hadsignificantbiocontroleffectsonrootrotofAtractylodeschinensis,withcontroleffectsof52.27%and68.19%,respectively.StudieshaveshownthatFusariumoxysporumcancauserootrotofAtractylodeschinensisandBacillussubtilisandBacillusvelezensishavepreventiveeffectontherootrotofAtractylodeschinensis.Keywords：Atractylodeschinensis;root...
