Designation:F2259−10(Reapproved2012)´1StandardTestMethodforDeterminingtheChemicalCompositionandSequenceinAlginatebyProtonNuclearMagneticResonance(1HNMR)Spectroscopy1ThisstandardisissuedunderthefixeddesignationF2259;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.ε1NOTE—Editorialchangesweremadetosubsections2.2and4.2inNovember2012.1.Scope1.1Thistestmethodcoversthedeterminationofthecom-positionandmonomersequenceofalginateintendedforuseinbiomedicalandpharmaceuticalapplicationsaswellasinTissueEngineeredMedicalProducts(TEMPs)byhigh-resolutionprotonNMR(1HNMR).Aguideforthecharacter-izationofalginatehasbeenpublishedasGuideF2064.1.2Alginate,alinearpolymercomposedofβ-D-mannuronate(M)anditsC-5epimerα-L-guluronate(G)linkedbyβ-(1—>4)glycosidicbonds,ischaracterizedbycalculatingparameterssuchasmannuronate/guluronate(M/G)ratio,gu-luronicacidcontent(G-content),andaveragelengthofblocksofconsecutiveGmonomers(thatis,NG>1).KnowledgeoftheseparametersisimportantforanunderstandingofthefunctionalityofalginateinTEMPformulationsandapplica-tions.Thistestmethodwillassistendusersinchoosingthecorrectalginatefortheirparticularapplication.AlginatemayhaveutilityasascaffoldormatrixmaterialforTEMPs,incellandtissueencapsulationapplications,andindrugdeliveryformulations.1.3ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.4Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:2E386PracticeforDataPresentationRelatingtoHigh-ResolutionNuclearMagneticResonance(NMR)Spec-troscopyF2064GuideforCharacterizationandTestingofAlgi...
