Designation:F895−11StandardTestMethodforAgarDiffusionCellCultureScreeningforCytotoxicity1ThisstandardisissuedunderthefixeddesignationF895;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethodisappropriateformaterialsinavarietyofshapesandformaterialsthatarenotnecessarilysterile.Thistestmethodwouldbeappropriateinsituationsinwhichtheamountofmaterialislimited.Forexample,smalldevicesorpowderscouldbeplacedontheagarandthepresenceofazoneofinhibitionofcellgrowthcouldbeexamined.1.1.1Thistestmethodisnotappropriateforleachablesthatdonotdiffusethroughagaroragarose.1.1.2Whiletheagarlayercanactasacushiontoprotectthecellsfromthespecimen,theremaybematerialsthataresufficientlyheavytocompresstheagarandpreventdiffusionortocausemechanicaldamagetothecells.Thistestmethodwouldnotbeappropriateforthesematerials.1.2TheL-929celllinewaschosenbecauseithasasignificanthistoryofuseinassaysofthistype.Thisisnotintendedtoimplythatitsuseispreferred,onlythattheL-929isanestablishedcellline,wellcharacterizedandreadilyavailable,thathasdemonstratedreproducibleresultsinseverallaboratories.1.3ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.4Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:2F748PracticeforSelectingGenericBiologicalTestMethodsforMaterialsandDevices2.2ATCCDocument:AmericanTypeCultureCollection,(ATCC)CatalogueofStrainsII3USPNegativeControlPlasticReferenceStandard43.SummaryofTestMethod3.1Cellculturesaregrowntoamonolayerinculturedishes.Themediumisaspiratedandreplacedwithanagar-containingmedium...
