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TM_E_2922_
_15
Designation:E292215Standard Guide forThe Use of Standard Test Methods and Practices forEvaluating Antibacterial Activity on Textiles1This standard is issued under the fixed designation E2922;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This guide provides users with an index of proceduresin the form of test methods,practices,and related internationaldocuments that are currently used in the textile industry fordetermining antibacterial properties of antimicrobial treatedtextile articles.This guide is not considered as all-inclusive forantimicrobial testing procedures related to textiles.1.2 This guide identifies some existing ASTM and otherindustry standard test methods applicable for testing theantibacterial performance on textiles and discusses optionswithin each method that have been used to address specificend-use performance expectations.1.3 This guide is intended to assist testing facilities indetermining which test methods are appropriate for whichtreated articles based on type of antimicrobial active involved(diffusible versus non-diffusible),nature of test fabric,andexpected end use.1.4 The test methods indicated in this guide should beperformed only by those trained in microbiological techniques,are familiar with textile antimicrobial agents and with the enduse exposures of the antimicrobial treated textile material.1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2E2149 Test Method for Determining the Antimicrobial Ac-tivity of Antimicrobial Agents Under Dynamic ContactConditionsE2180 Test Method for Determining the Activity of Incor-porated Antimicrobial Agent(s)In Polymeric or Hydro-phobic MaterialsE2722 Test Method for Using Seeded-Agar for the Screen-ing Assessment of Antimicrobial Activity in Fabric andAir Filter MediaE2756 Terminology Relating to Antimicrobial and AntiviralAgents2.2 AATCC Standards:3AATCC Test Method 90:Antibacterial Activity Assessmentof Textile Materials:Agar Plate Method.American Asso-ciation of Textile Chemists and Colorists,RTP,NCAATCC Test Method 100:Antibacterial Finishes on Fabrics,Evaluation of.American Association of Textile Chemistsand Colorists,RTP,NCAATCC Test Method 147:Antibacterial Activity Assess-ment of Textile Materials:Parallel Streak Method.Ameri-can Association of Textile Chemists and Colorists,RTP,NC2.3 ISO Standards:4ISO 20743 Textiles Determination of Antibacterial Activ-ity of Antibacterial Finished ProductsISO 22196 Plastics Measurement of Antibacterial Activityon Plastics Surfaces2.4 JIS Standards:5JIS L 1902 Testing forAntibacterialActivity and Efficacy onTextile ProductsJIS Z 2801 Antimicrobial Products Test for AntimicrobialActivity and Efficacy2.5 Other Standards:SNV 195920 Examination of the Antimicrobial Effect ofImpregnated Textiles by the Agar Diffusion Test4IBRG TEX13/005/1.0 Quantitative Method for EvaluatingBactericidal Activity of Textiles and Porous Materials andArticles1This guide is under the jurisdiction of ASTM Committee E35 on Pesticides,Antimicrobials,and Alternative Control Agents and is the direct responsibility ofSubcommittee E35.15 on Antimicrobial Agents.Current edition approved May 1,2015.Published June 2015.DOI:10.1520/E2922152For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3Available from AATCC 1 Davis Dr Research Triangle Park,NC 27709-2215USA.http:/www.aatcc.org/4Available from International Organization for Standardization(ISO),1,ch.dela Voie-Creuse,CP 56,CH-1211 Geneva 20,Switzerland,http:/www.iso.org.5Available from Japanese Industrial Standards Committee(JIS)1-3-1Kasumigaseki,Chiyoda-ku,Tokyo 100-8901,JAPAN.http:/www.jisc.go.jpCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 3.Terminology3.1For definitions of terms used in this Guide see Termi-nology E2756.4.Significance and Use4.1 Antimicrobial agents are routinely used for treatingtextile materials for the reduction of biodeterioration andbacterial odor generation.Furthermore,textiles are treated toprevent or limit microbial cross-contamination in healthcaresettings.4.2 Antimicrobial agents used in textiles will vary withregard to their broad-spectrum effectiveness,biostatic/biocidalproperties,and binding properties in or on particular substrates.When selecting antibacterial test methods as the sole