Designation:E3029−15StandardPracticeforDeterminingRelativeSpectralCorrectionFactorsforEmissionSignalofFluorescenceSpectrometers1ThisstandardisissuedunderthefixeddesignationE3029;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thispractice(1)2describesthreemethodsfordetermin-ingtherelativespectralcorrectionfactorsforgrating-basedfluorescencespectrometersintheultraviolet-visiblespectralrange.Thesemethodsareintendedforinstrumentswitha0°/90°transmittingsamplegeometry.Eachmethodusesdif-ferenttypesoftransferstandards,including1)acalibratedlightsource(CS),2)acalibrateddetector(CD)andacalibrateddiffusereflector(CR),and3)certifiedreferencematerials(CRMs).Thewavelengthregioncoveredbythedifferentmethodsrangesfrom250to830nmwithsomemethodshavingabroaderrangethanothers.Extendingthesemethodstothenearinfrared(NIR)beyond830nmwillbediscussedbriefly,whereappropriate.Thesemethodsweredesignedforscanningfluorescencespectrometerswithasinglechanneldetector,butcanalsobeusedwithamultichanneldetector,suchasadiodearrayoraCCD.1.2ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:3E131TerminologyRelatingtoMolecularSpectroscopyE388TestMethodforWavelengthAccuracyandSpectralBandwidthofFluorescenceSpectrometersE578TestMethodforLinearityofFluorescenceMeasuringSystemsE2719GuideforFluorescence—InstrumentCalibrationandQualification3.SignificanceandUse(Intro)3.1Calibrationoftheresponsivityofthedetectionsystemforemission(EM)asafunctionofEMwavelength(λEM),also...
