ASTM_C_1338_-_14.pdf

Designation:C133814Standard Test Method forDetermining Fungi Resistance of Insulation Materials andFacings1This standard is issued under the fixed designation C1338;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method covers the determination of the abilityof new insulation materials and their facings to resist fungalgrowth.1.2 The values stated in inch-pound units are to be regardedas standard.The values given in parentheses are mathematicalconversions to SI units that are provided for information onlyand are not considered standard.1.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Significance and Use2.1 The type of materials used in the manufacture ofinsulation products and the type of membrane used to facethese products can sometimes affect fungi sustenance in thepresence of high humidity.2.2 This test method is used to determine the relative abilityof an insulation and its facing to resist fungal growth underconditions favorable for their development.2.3 This test method uses a comparative material to deter-mine the relative ability of a material to resist fungal growth.Insome specialized product areas,it is required that no growthtake place.In such cases,the use of the comparative materialis omitted and the pass/fail criterion is based upon growth.3.Apparatus3.1 GlasswareSterile disposable petri dishes,4 or 6 in.(100 or 150 mm)by 0.6 or 0.75 in.(15 or 20 mm)in size arepreferred.For larger specimens,trays of borosilicate glass orbaking dishes up to 16 by 20 in.(400 by 600 mm)in size maybe used.3.2 Environmental Chamber or CabinetEquipment forthis test method shall maintain a temperature of 82.4 to 86F(28 to 30C)and a relative humidity of 95%(64%).Provisions shall be made to prevent condensation from drip-ping on the test specimen.There shall be free circulation of airaround the test chamber.3.3 AtomizerA chromatography atomizer capable of pro-viding 100 000 6 20 000 spores/in.2(15 000 6 3000 spores/cm2)shall be used for inoculation.3.4 Autoclavable Biohazard Bags,or metal pan able towithstand autoclaving.4.Reagents and Materials4.1 Purity of WaterUnless otherwise specified,referencesto water shall be understood to mean sterile distilled water orwater of equal purity.4.2 Inoculum:FungiATCC2Aspergillus niger9642Aspergillus versicolor11 730Penicillium funiculosum11 797Chaetomium globosum6205Aspergillus flavus96434.3 CulturesMaintain cultures of the Aspergillus fungiseparately on Czapek Dox agar(see Note 1).Culture theChaetomium globosum on strips of cellulose filter paper on thesurface of Czapek Dox agar.Maintain the Penicillium fungi onSabouraud Dextrose agar.The stock cultures may be kept fornot more than 4 months at 43 6 7F(6 6 4C)at which timesubcultures shall be made,and new stocks selected from thesubcultures.If genetic or physiological changes occur,obtainnew cultures.Incubate subcultures used for preparing newstock cultures or the spore suspension at 86 6 4F(30 6 2C)for 5 days or longer.NOTE1This media is readily available from any science/microbiological supply house.1This test method is under the jurisdiction ofASTM Committee C16 on ThermalInsulation and is the direct responsibility of Subcommittee C16.31 on Chemical andPhysical Properties.Current edition approved Jan.1,2014.Published February 2014.Originallyapproved in 1996.Last previous edition approved in 2008 as C133808.DOI:10.1520/C1338-14.2The sole source of supply of the cultures known to the committee at this timeis American Type Culture Collection(ATCC),12301 Parklawn Drive,Rockville,MD 20852.If you are aware of alternative suppliers,please provide this informationto ASTM International Headquarters.Your comments will receive careful consid-eration at a meeting of the responsible technical committee,1which you may attend.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 5.Specimens5.1 Viability SpecimensDetermine the viability of thespore suspension during incubation with these controls:witheach daily group of tests,place one piece of sterilized What-man 500 filter paper,1 in.2(6.4 cm2)on each of two preparedhardened Czapek Dox agar specimens in separate petri dishes.Prepare a third petri dish with Sabouraud Dextrose agar.5.2 Comparative MaterialAwhite birch tongue depressor,0.75 by 6 in.(20 by 150 mm)or southern yellow pine is thecomparative item to determine the relative growth on speci-mens being tested.The choice of comparative item should benoted.Refer to the appropriate materials