ASTM_D_2574_-_16.pdf

Designation:D257416Standard Test Method forResistance of Emulsion Paints in the Container to Attack byMicroorganisms1This standard is issued under the fixed designation D2574;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope*1.1 This test method covers the determination of the relativeresistance of emulsion paints to attack in the container bymicroorganisms.1.2 The values stated in SI units are to be regarded as thestandard.The values given in parentheses are for informationonly.1.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D5588 Test Method for Determination of the MicrobialCondition of Paint,Paint Raw Materials,and Plant Areas3.Summary of Test Method3.1 This test method is designed to challenge samples of oneor more paints containing various levels of one or morebiocides with a known amount of bacteria and rate the abilityof the test paint(s)to control the“contamination.”4.Significance and Use4.1 Spoilage of paint in the container can result inputrefaction,lowered pH,gas formation,and decrease inviscosity.This test method provides a standard procedure forthe evaluation of the resistance of emulsion paints to microbialdeterioration.The results should enable:(1)the paint manu-facturer to select an effective preservative and(2)the supplierof preservatives to evaluate the performance in emulsion paintsof competitive and developmental preservatives.4.2 This test method should be used preferably by personswho have had basic microbiological training.NOTE1The reliability of the results obtained from this test method isextremely dependent on the techniques employed.Improper techniquescan result in a sterile sample appearing to be contaminated,and evenworse,a contaminated sample appearing to be sterile(see also Note 2).Itis recommended that you consult with your biocide supplier,raw materialsupplier,or an independent testing laboratory to confirm questionableresults.Formulation and raw materialsquality may also vary and therebyaffect the test results.5.Apparatus and Materials5.1 Balance,capable of weighing to 0.10 g.5.2 Incubator,or other device capable of maintaining aconstant temperature between 28 and 32C.5.3 Refrigerator,maintained at 10 to 13C.5.4 Screwcap Borosilicate Test Tubes,125 by 15-mm.5.5 Borosilicate Flasks,1-L.5.6 Screwcap Bottles,150-mL.5.7 Autoclave,capable of producing 103 kPa(15 psi)ofsteam pressure at 121C and maintaining it for a minimum of15 min.An autoclave is not necessary if prepared agar slantsare used.5.8 Pipettes or an Automatic Pipettor,sterile,1-mL,withsterile disposable pipette tips for 1 mL.5.9 Petri Dishes,sterile.5.10 Dehydrated Tryptic Soy Agar(TSA),medium,or pre-prepared slants,plates,and broth tubes.35.11 Swabs,sterile cotton.1This test method is under the jurisdiction of ASTM Committee D01 on Paintand Related Coatings,Materials,and Applications and is the direct responsibility ofSubcommittee D01.28 on Biodeterioration.Current edition approved June 1,2016.Published July 2016.Originally approvedin 1967.Last previous edition approved in 2012 as D2574 06(2012).DOI:10.1520/D2574-16.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3Available from microbiological supply companies.Media with TTC indicatordye may be used.In general,the TTC helps visualize contamination,but it has beenreported on occasion to inhibit the growth of some bacteria.Interferences frompigments in materials being tested may make the color change difficult to see.Ifself-prepared plates are used with the TTC indicator,0.01%TTC indicator shouldbe used and it must be added after autoclaving.*A Summary of Changes section appears at the end of this standardCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 5.12 Laminar Flow Hood,Sterile Room,or at Least aLaboratory Testing Area,relatively clean,free of blowing dustand dirt,etc.,which can be used for streaking plates.5.13 Antiseptic Solution,to help maintain sterility of testingarea surfaces(4.12)(for example,70%ethanol solution).5.14 A minimum of 235 mL(12pt)of each paint sampleunder test(pre-loaded with biocide).5.15 Aminimum of 475 mL(1 pt)of paint identical to 5.14,but containing no biocide.5.16 Twenty-four Hour Cultures of a Pseudomonas sp.(forexample,Pseudomonas aeruginosa ATCC#10145)and anEnterobacter sp